Deeper insight into the effect of salinity on the relationship of enzymatic activity, microbial community and key metabolic pathway during the anaerobic digestion of high strength organic wastewater

被引：35

作者：

Yin, Yijang ^{[1
]}

Zhang, Zengshuai ^{[1
,2
,3
]}

Yang, Kunlun ^{[1
,2
,3
]}

Gu, Peng ^{[1
,2
,3
]}

Liu, Shiguang ^{[1
]}

Jia, Yifan ^{[1
]}

Zhang, Zhaochang ^{[1
]}

Wang, Tao ^{[4
]}

Yin, Jianqi ^{[5
]}

Miao, Hengfeng ^{[1
,2
,3
,6
,7
]}

机构：

[1] Jiangnan Univ, Sch Environm & Civil Engn, Wuxi 214122, Peoples R China

[2] Jiangnan Univ, Jiangsu Key Lab Anaerob Biotechnol, Wuxi 214122, Peoples R China

[3] Jiangnan Univ, Jiangsu Engn Lab Biomass Energy & Carbon Reduct Te, Wuxi 214122, Peoples R China

[4] Wuxi Univ, Sch Environm Engn, Wuxi 214105, Peoples R China

[5] Columbia Univ, Dept Earth & Environm Engn, New York, NY 10027 USA

[6] Suzhou Univ Sci & Technol, Water Treatment Technol & Mat Innovat Ctr, Suzhou 215009, Peoples R China

[7] Jiangnan Univ, Wuxi 214122, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 363卷

关键词：

Salinity; Anaerobic digestion; Enzyme activity; Functional genes; Microbial communities; METHANE PRODUCTION; AMMONIA; REMOVAL;

D O I：

10.1016/j.biortech.2022.127978

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The threshold salt concentration to inhibit the anaerobic digestion (AD) has been intensively investigated, but its insight mechanism is not fully revealed. Therefore, this study systematically investigated the effect of salinity on acidogenesis and methanogenesis and its mechanism. Results showed that low salinity level (i.e. 0.6%) had stimulatory effect on volatile fatty acids (VFA) and methane production, while significant inhibition was observed with further increased salinity. Moreover, high salinity limited the butyric acid degradation at acido-genesis process. The decreases of enzymes (AK and PTA) activity and functional genes (ackA, pta and ACOX) expression that related to 8-oxidation explained the butyric acid accumulation at high salinity levels. Microbial community analysis revealed high salinity levels significantly inhibited the proliferation of Syntrophomonas sp., which are known to be associated with butyric acid degradation. Similarly, the relative abundance of acetoclastic methanogen (Methanothrix sp.) and methylotrophic methanogen (Methanolinea sp.) significantly decreased at salinity condition.

引用

页数：8

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