Safety Assessment by Multiphoton Fluorescence/Second Harmonic Generation/Hyper-Rayleigh Scattering Tomography of ZnO Nanoparticles Used in Cosmetic Products

被引：70

作者：

Darvin, M. E. ^{[1
]}

Koenig, K. ^{[2
]}

Kellner-Hoefer, M. ^{[2
]}

Breunig, H. G. ^{[2
]}

Werncke, W. ^{[1
]}

Meinke, M. C. ^{[1
]}

Patzelt, A. ^{[1
]}

Sterry, W. ^{[1
]}

Lademann, J. ^{[1
]}

机构：

[1] Charite, Dept Dermatol Venereol & Allergol, Ctr Expt & Cutaneous Physiol CCP, DE-10117 Berlin, Germany

[2] JenLab GmbH, Jena, Germany

来源：

SKIN PHARMACOLOGY AND PHYSIOLOGY | 2012年 / 25卷 / 04期

关键词：

Skin; Penetration; Sunscreens; Ultraviolet filter; Zinc oxide; Stratum corneum; ZINC-OXIDE NANOPARTICLES; HUMAN SKIN; IN-VIVO; PERCUTANEOUS UPTAKE; TITANIUM-DIOXIDE; STRATUM-CORNEUM; DRUG-DELIVERY; FREE-RADICALS; SUNSCREEN; PENETRATION;

D O I：

10.1159/000338976

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Zinc oxide nanoparticles (ZnO NPs) are commonly used as UV filters in commercial sunscreen products. Their penetration into the skin is intensively discussed in the literature. In the present in vivo study, penetration of ZnO NPs (30 nm in size) into human skin was investigated by multiphoton tomography. Based on the non-linear effects of a second harmonic generation and hyper-Rayleigh scattering, the distribution of ZnO NPs in the horny layers of the epidermis, as well as the furrows, wrinkles and orifice of the hair follicles was analyzed. This method permitted distinguishing between the particulate and dissolved forms of Zn. A detection limit of 0.08 fg/mu m(3) was estimated. Taking advantage of this sensitivity, it was clearly shown that ZnO NPs penetrate only into the outermost layers of stratum corneum, furrows and into the orifices of the hair follicles and do not reach the viable epidermis. Copyright (C) 2012 S. Karger AG, Basel

引用

页码：219 / 226

页数：8

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