Application of shRNA-containing herpes simplex virus type 1 (HSV-1)-based gene therapy for HSV-2-induced genital herpes

被引：3

作者：

Liu, Zhihong ^{[1
,2
]}

Xiang, Yang ^{[3
]}

Wei, Zhun ^{[2
,4
]}

Yu, Bo ^{[2
,4
,5
,6
]}

Shao, Yong ^{[2
,4
,5
,6
]}

Zhang, Jie ^{[2
,4
,5
,6
]}

Yang, Hong ^{[2
,4
,5
,7
]}

Li, Manmei ^{[8
]}

Guan, Ming ^{[9
]}

Wan, Jun ^{[2
,10
]}

Zhang, Wei ^{[2
,4
,5
]}

机构：

[1] Peking Univ, Shenzhen Hosp, Dept Obstet & Gynecol, Shenzhen 518036, Guangdong, Peoples R China

[2] Shenzhen PKU HKUST Med Ctr, Biomed Res Inst, Shenzhen 518036, Guangdong, Peoples R China

[3] Fudan Univ, Huashan Hosp, Dept Gen Surg, Shanghai 200040, Peoples R China

[4] Shenzhen PKU HKUST Med Ctr, Shenzhen Key Lab Translat Med Dermatol, Shenzhen 518036, Guangdong, Peoples R China

[5] Peking Univ, Shenzhen Hosp, Shenzhen Key Discipline Dermatol, Shenzhen 518036, Guangdong, Peoples R China

[6] Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen 518036, Guangdong, Peoples R China

[7] Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen 518036, Guangdong, Peoples R China

[8] Ji Nan Univ, Coll Pharm, JNU HKUST Joint Lab, Jinan, Guangdong, Peoples R China

[9] Fudan Univ, Huashan Hosp, Cent Lab, Dept Lab Med, Shanghai 200040, Peoples R China

[10] Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2013年 / 193卷 / 02期

关键词：

Herpes simplex virus type 1 (HSV-1) vector; Herpes simplex virus type 2 (HSV-2); Genital herpes; IMMUNOGENICITY; GLYCOPROTEINS; PROTECTION; INFECTION; IMMUNITY; NEURONS; HSV-2; MODEL; MICE;

D O I：

10.1016/j.jviromet.2013.06.037

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the severity of vaginal disease. HSV-2 shRNA-containing HSV-1 was applied intravaginally daily between 8 and 14 days after HSV-2 challenge. Delivery of HSV-2 shRNA-containing HSV-1 had no effect on the onset of disease and acute virus shedding in animals, but resulted in a significant reduction in both the cumulative recurrent lesion days and the number of days with recurrent disease. Around half of the animals in the HSV-2 shRNA group did not develop recurrent disease 100 days post HSV-2 infection. In conclusion, HSV-2 shRNA-containing HSV-1 particles are effective in reducing the recurrence of genital herpes caused by HSV-2. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：353 / 358

页数：6

共 24 条

[11] The quantity of latent viral DNA correlates with the relative rates at which herpes simplex virus types 1 and 2 cause recurrent genital herpes outbreaks [J].

Lekstrom-Himes, JA ;

Pesnicak, L ;

Straus, SE .

JOURNAL OF VIROLOGY, 1998, 72 (04) :2760-2764

[12] Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity [J].

Malin, Sacha A. ;

Davis, Brian M. ;

Molliver, Derek C. .

NATURE PROTOCOLS, 2007, 2 (01) :152-160

[13] Immunization with Herpes Simplex Virus 2 (HSV-2) Genes plus Inactivated HSV-2 Is Highly Protective against Acute and Recurrent HSV-2 Disease [J].

Morello, Christopher S. ;

Levinson, Michael S. ;

Kraynyak, Kimberly A. ;

Spector, Deborah H. .

JOURNAL OF VIROLOGY, 2011, 85 (07) :3461-3472

[14]

Nahmias A J, 1990, Scand J Infect Dis Suppl, V69, P19

[15] Protective immunity against HSV-2 in the mouse vagina [J].

Parr, MB ;

Parr, EL .

JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1997, 36 (1-2) :77-92

[16]

PARR MB, 1994, LAB INVEST, V70, P369

[17] Reduced Expression of Brain-Enriched microRNAs in Glioblastomas Permits Targeted Regulation of a Cell Death Gene [J].

Skalsky, Rebecca L. ;

Cullen, Bryan R. .

PLOS ONE, 2011, 6 (09)

[18] VACCINATION WITH RECOMBINANT HERPES-SIMPLEX VIRUS GLYCOPROTEINS - PROTECTION AGAINST INITIAL AND RECURRENT GENITAL HERPES [J].

STANBERRY, LR ;

BERNSTEIN, DI ;

BURKE, RL ;

PACHL, C ;

MYERS, MG .

JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (05) :914-920

[19]

Tabbaa S, 2005, Folia Morphol (Warsz), V64, P273

[20] Herpes simplex virus [J].

Taylor, TJ ;

Brockman, MA ;

McNamee, EE ;

Knipe, DM .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2002, 7 :D752-D764

← 1 2 3 →