Screening active components acting on α1A adrenergic receptors from agrimony using a Sprague-Dawley rat prostate cell membrane chromatography online coupled HPLC/MS method

被引：22

作者：

Han, Shengli ^{[1
]}

Zhang, Peng ^{[2
]}

Wei, Fen ^{[1
]}

Wang, Sicen ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Sch Med, Xian 710061, Peoples R China

[2] Shaanxi Prov Peoples Hosp, Dept Pharm, Xian 710068, Peoples R China

来源：

ANALYTICAL METHODS | 2012年 / 4卷 / 10期

基金：

中国国家自然科学基金;

关键词：

GROWTH-FACTOR RECEPTOR; EXPRESSION; EUPATORIA; EXTRACTS;

D O I：

10.1039/c2ay25703c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Agrimony is a traditional Chinese medicine and a herb of Agrimonia pilosa Ledeb belonging to the Rosaceae family. Many pharmacological effects of agrimony have been demonstrated in previous studies. Few studies have screened the active compounds in this complex product for chronic prostatitis. In this study, a two dimensional online method was established. A Sprague-Dawley (SD) rat prostate CMC-online-HPLC/MS method was used to screen, analyze, and identify active compounds acting on alpha(1) adrenergic receptor from agrimony. Via this online method agrimonolide was screened, analyzed, and identified as the active compound acting on alpha(1) adrenergic receptor. Thus, agrimonolide is a potential alpha(1) adrenergic receptor antagonist and requires further study.

引用

页码：3351 / 3357

页数：7

共 24 条

[1] Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines [J].

Chen, Xiaofei ;

Cao, Yan ;

Lv, Diya ;

Zhu, Zhenyu ;

Zhang, Junping ;

Chai, Yifeng .

JOURNAL OF CHROMATOGRAPHY A, 2012, 1242 :67-74

[2]

Fang GuiZhen Fang GuiZhen, 2002, Journal of Northeast Forestry University, V30, P36

[3]

Ferguson SSG, 2001, PHARMACOL REV, V53, P1

[4] Effect of in vitro digestion upon the antioxidant capacity of aqueous extracts of Agrimonia eupatoria, Rubus idaeus, Salvia sp and Satureja montana [J].

Giao, Maria S. ;

Gomes, Susana ;

Madureira, Ana R. ;

Faria, Ana ;

Pestana, Diogo ;

Calhau, Conceicao ;

Pintado, Manuela E. ;

Azevedo, Isabel ;

Xavier Malcata, F. .

FOOD CHEMISTRY, 2012, 131 (03) :761-767

[5] Actions of the traditional anti-diabetic plant, Agrimony eupatoria (agrimony):: effects on hyperglycaemia, cellular glucose metabolism and insulin secretion [J].

Gray, AM ;

Flatt, PR .

BRITISH JOURNAL OF NUTRITION, 1998, 80 (01) :109-114

[6] Screening active compounds acting on the epidermal growth factor receptor from Radix scutellariae via cell membrane chromatography online coupled with HPLC/MS [J].

He, Huaizhen ;

Han, Shengli ;

Zhang, Tao ;

Zhang, Jie ;

Wang, Sicen ;

Hou, Jingjing .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2012, 62 :196-202

[7]

He L. C., 1996, NEW PROG BIOMED CHRO, V3, P8

[8] Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface [J].

He, LC ;

Yang, GD ;

Geng, XD .

CHINESE SCIENCE BULLETIN, 1999, 44 (09) :826-831

[9] A vascular smooth muscle/cell membrane chromatography-offline-gas chromatography/mass spectrometry method for recognition, separation and identification of active components from traditional Chinese medicines [J].

Hou, Xiaofang ;

Zhou, Mingzhe ;

Jiang, Qiao ;

Wang, Sicen ;

He, Langchong .

JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (42) :7081-7087

[10] In vitro antioxidant activity of acetone extracts from Chinese herb agrimony leaves [J].

Jin, Zhe-xiong ;

Wang, Bao-Qing .

JOURNAL OF MEDICINAL PLANTS RESEARCH, 2011, 5 (25) :6005-6010

← 1 2 3 →