Enzyme-regulated unmodified gold nanoparticle aggregation: a label free colorimetric assay for rapid and sensitive detection of adenosine deaminase activity and inhibition

被引：24

作者：

Zhang, Liangliang ^{[1
]}

Zhao, Jingjin ^{[1
]}

Jiang, Jianhui ^{[1
]}

Yu, Ruqin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 89期

关键词：

DEFICIENCY; BINDING;

D O I：

10.1039/c2cc36240f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L-1

引用

页码：10996 / 10998

页数：3

共 16 条

[1]

Alunni S, 2008, J ENZYM INHIB MED CH, V23, P182, DOI [10.1080/14756360701475233, 10.1080/14756360701475233 ]

[2] Adenosine Deaminase Deficiency: Unanticipated Benefits from the Study of a Rare Immunodeficiency [J].

Blackburn, Michael R. ;

Thompson, Linda F. .

JOURNAL OF IMMUNOLOGY, 2012, 188 (03) :933-935

[3] Probing BSA binding to citrate-coated gold nanoparticles and surfaces [J].

Brewer, SH ;

Glomm, WR ;

Johnson, MC ;

Knag, MK ;

Franzen, S .

LANGMUIR, 2005, 21 (20) :9303-9307

[4] Small-molecule screening made simple for a difficult target with a signaling nucleic acid aptamer that reports on deaminase activity [J].

Elowe, Nadine H. ;

Nutiu, Razvan ;

Allah-Hassani, Abdellah ;

Cechetto, Jonathan D. ;

Hughes, Donald W. ;

Li, Yingfu ;

Brown, Eric A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (34) :5648-5652

[5] Isothermal titration calorimetry studies on the binding of DNA bases and PNA base monomers to gold nanoparticles [J].

Gourishankar, A ;

Shukla, S ;

Ganesh, KN ;

Sastry, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (41) :13186-13187

[6]

Gupta Bharat Kumar, 2010, J Clin Med Res, V2, P79, DOI 10.4021/jocmr2010.03.280w

[7] Genotype is an important determinant of phenotype in adenosine deaminase deficiency [J].

Hershfield, MS .

CURRENT OPINION IN IMMUNOLOGY, 2003, 15 (05) :571-577

[8] ENZYME AND MEMBRANE MARKERS IN LEUKEMIA - RECENT DEVELOPMENTS [J].

HOFFBRAND, AV ;

JANOSSY, G .

JOURNAL OF CLINICAL PATHOLOGY, 1981, 34 (03) :254-262

[9]

KLOCKARS M, 1985, CLIN CHEM, V31, P155

[10] The Fabrication of Stable Gold Nanoparticle-Modified Interfaces for Electrochemistry [J].

Liu, Guozhen ;

Luais, Erwann ;

Gooding, J. Justin .

LANGMUIR, 2011, 27 (07) :4176-4183

← 1 2 →