Microsatellite DNA markers in the giant freshwater prawn, Macrobrachium rosenbergii:: a tool for genetic analysis

被引：7
|
作者：
Divu, Damodaran [1 ]
Karunasagar, Iddya [1 ]
Karunasagar, Indrani [1 ]
机构：
[1] Karnataka Vet Anim & Fisheries Sci Univ, Coll Fisheries, Dept Fishery Microbiol, Mangalore 575002, India
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 05期
关键词：
breeding; genetic Studies; heterozygosity; Macrobrachium rosenbergii; microsatellite; primer;
D O I：
10.1111/j.1755-0998.2008.02148.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eight microsatellite loci were identified and characterized in the commercially important giant freshwater prawn, Macrobrachium rosenbergii. The microsatellite loci were detected by the random screening for dinucleotide repeat units within a partial genomic library developed for the species with biotinylated probes (CA)(15), (AT)(15), and (GA)(15). All the eight loci were found to be polymorphic. The number of alleles and observed heterozygosities per locus ranged between three to 16 and 0.22 to 0.71, respectively. These microsatellite markers will be useful for the conservation and management of wild and cultured stocks and population genetic studies of freshwater prawn.
引用
收藏
页码：1040 / 1042
页数：3
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