Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain

被引:48
作者
Vilela, Leonardo de Figueiredo [1 ]
Gomes de Araujo, Veronica Parente [1 ]
Paredes, Raquel de Sousa [1 ]
da Silva Bon, Elba Pinto [1 ]
Goncalves Torres, Fernando Araripe [2 ]
Neves, Bianca Cruz [1 ]
Araujo Eleutherio, Elis Cristina [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Chem, Dept Biochem, Rio De Janeiro, Brazil
[2] Univ Brasilia, Inst Biol, Dept Cellular Biol, Brasilia, DF, Brazil
关键词
Evolutionary engineering; Xylose isomerase; Saccharomyces cerevisiae; TAL1; Xylose; Ethanol; PENTOSE-PHOSPHATE PATHWAY; GLUCOSE; EXPRESSION; METABOLISM; GENES; RATES; PCR;
D O I
10.1186/s13568-015-0102-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have recently demonstrated that heterologous expression of a bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia enabled a laboratorial Saccharomyces cerevisiae strain to ferment xylose anaerobically, without xylitol accumulation. However, the recombinant yeast fermented xylose slowly. In this study, an evolutionary engineering strategy was applied to improve xylose fermentation by the xylA-expressing yeast strain, which involved sequential batch cultivation on xylose. The resulting yeast strain co-fermented glucose and xylose rapidly and almost simultaneously, exhibiting improved ethanol production and productivity. It was also observed that when cells were grown in a medium containing higher glucose concentrations before being transferred to fermentation medium, higher rates of xylose consumption and ethanol production were obtained, demonstrating that xylose utilization was not regulated by catabolic repression. Results obtained by qPCR demonstrate that the efficiency in xylose fermentation showed by the evolved strain is associated, to the increase in the expression of genes HXT2 and TAL1, which code for a low-affinity hexose transporter and transaldolase, respectively. The ethanol productivity obtained after the introduction of only one genetic modification and the submission to a one-stage process of evolutionary engineering was equivalent to those of strains submitted to extensive metabolic and evolutionary engineering, providing solid basis for future applications of this strategy in industrial strains.
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页数:7
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