High glucose-induced endothelial microparticles increase adhesion molecule expression on endothelial cells

被引：9
作者：
Hijmans, Jamie G. [1 ]
Bammert, Tyler D. [1 ]
Stockelman, Kelly A. [1 ]
Reiakvam, Whitney R. [1 ]
Greiner, Jared J. [1 ]
DeSouza, Christopher A. [1 ]
机构：
[1] Univ Colorado, Integrat Vasc Biol Lab, Dept Integrat Physiol, 354 UCB,1725 Pleasant St, Boulder, CO 80309 USA
来源：
DIABETOLOGY INTERNATIONAL | 2019年 / 10卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Glucose; Endothelial cells; Microparticles; Cell adhesion molecules; E-SELECTIN; NANOPARTICLES; COMPLICATIONS; DYSFUNCTION; VCAM-1; ICAM-1;
D O I：
10.1007/s13340-018-0375-x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The experimental aim of this study was to determine, in vitro, the effects of glucose-induced EMPs on endothelial cell expression of E-selectin, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 and platelet cell adhesion molecule-1 (PECAM-1). Human umbilical vein endothelial cells (HUVECs) were cultured (3rd passage) and plated in 6-well plates at a density of 5.0x10(5) cells/condition. HUVECs were incubated with media containing either 25mM d-glucose (concentration representing a hyperglycemic state) or 5mM d-glucose (normoglycemic condition) for 48h to generate EMPs. EMP identification (CD144(+)) and concentration were determined by flow cytometry. HUVECs (3 x 10(6) cells/condition) were treated with either high glucose-derived EMPs (hgEMPs) or normal glucose-derived (ngEMPs) for 24h and surface expression of E-selectin (CD62E-PE), ICAM-1 (CD54-FITC), VCAM-1 (CD106-APC) and PECAM-1 (CD31-BV) was assessed by flow cytometry and reported as mean fluorescent intensity (MFI). Hyperglycemic-derived EMPs induced significantly higher surface expression of E-selectin (2614 +/- 132 vs. 2010 +/- 204MFI), ICAM-1 (2110 +/- 81 vs. 1688 +/- 152MFI), VCAM-1 (3589 +/- 431 vs. 2134 +/- 386) and PECAM-1 (4237 +/- 395 vs. 2525 +/- 269MFI) on endothelial cells than EMPs from normoglycemic conditions. Microparticle-induced cell adhesion molecule expression provides potential novel mechanistic insight regarding the accelerated risk of atherosclerotic vascular disease associated with hyperglycemia.
引用
收藏
页码：143 / 147
页数：5
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