The cloning, characterization and functional analysis of a gene encoding an acetyl-CoA acetyltransferase involved in triterpene biosynthesis in Ganoderma lucidum

被引：24

作者：

Fang, Xing ^{[1
]}

Shi, Liang ^{[1
]}

Ren, Ang ^{[1
]}

Jiang, Ai-Liang ^{[1
]}

Wu, Feng-Li ^{[1
]}

Zhao, Ming-Wen ^{[1
]}

机构：

[1] Nanjing Agr Univ, Dept Microbiol, Key Lab Microbiol Engn Agr Environm, Minist Agr,Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China

来源：

MYCOSCIENCE | 2013年 / 54卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Function; Over-expression; CYTOSOLIC ACETOACETYL-COENZYME; THIOLASE; EXPRESSION; PATHWAY;

D O I：

10.1016/j.myc.2012.09.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To better understand the functions of key genes involved in triterpene biosynthesis in Ganoderma lucidum, we cloned and characterized an acetyl coenzyme A acetyltransferase gene (AACT), designated Ganoderma lucidum AACT (Gl-aact). The open reading frame (ORF) of Gl-aact encoded a 417-amino acid polypeptide with a theoretical pI of 8.89. The Gl-AACT sequence was highly homologous to those of other fungal AACTs. Meanwhile, analysis of the Gl-aact expression profile revealed a positive correlation between Gl-aact transcript abundance and triterpene content changes during the development of G. lucidum. The treatment of mycelia with exogenous methyl jasmonate (MeJA) caused the accumulation of Gl-aact messenger RNA. Gl-aact over-expressing transformants were obtained by Agrobacterium tumefaciens-mediated transformation (ATMT), and triterpene production was observed to be increased when Gl-aact was over-expressed. Our results suggest that this enzyme may play an important role in triterpene biosynthesis. (C) 2012 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved.

引用

页码：100 / 105

页数：6

共 25 条

[1] The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase is essential for viability, and a single Leu-to-Pro mutation in a conserved sequence leads to thermosensitivity [J].