The L-type calcium ion channel Cch1 affects ascospore discharge and mycelial growth in the filamentous fungus Gibberella zeae (anamorph Fusarium graminearum)

被引:62
作者
Hallen, Heather E. [1 ]
Trail, Frances [1 ,2 ]
机构
[1] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Plant Pathol, E Lansing, MI 48824 USA
关键词
D O I
10.1128/EC.00248-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cch1, a putative voltage-gated calcium ion channel, was investigated for its role in ascus development in Gibberella zeae. Gene replacement mutants of CCH1 were generated and found to have asci which did not forcibly discharge spores, although morphologically ascus and ascospore development in the majority of asci appeared normal. Additionally, mycelial growth was significantly slower, and sexual development was slightly delayed in the mutant; mutant mycelia showed a distinctive fluffy morphology, and no cirrhi were produced. Wheat infected with Delta cch1 mutants developed symptoms comparable to wheat infected with the wild type; however, the mutants showed a reduced ability to protect the infected stalk from colonization by saprobic fungi. Transcriptional analysis of gene expression in mutants using the Affymetrix Fusarium microarray showed 2,449 genes with significant, twofold or greater, changes in transcript abundance across a developmental series. This work extends the role of CCH1 to forcible spore discharge in G. zeae and suggests that this channel has subtle effects on growth and development.
引用
收藏
页码:415 / 424
页数:10
相关论文
共 59 条
[1]  
*AFF, 2004, GENECHIP EXPR AN
[2]   Calcium signalling: Dynamics, homeostasis and remodelling [J].
Berridge, MJ ;
Bootman, MD ;
Roderick, HL .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529
[3]   The versatility and universality of calcium signalling [J].
Berridge, MJ ;
Lipp, P ;
Bootman, MD .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2000, 1 (01) :11-21
[4]   Calcineurin is essential for Candida albicans survival in serum and virulence [J].
Blankenship, JR ;
Wormley, FL ;
Boyce, MK ;
Schell, WA ;
Filler, SG ;
Perfect, JR ;
Heitman, J .
EUKARYOTIC CELL, 2003, 2 (03) :422-430
[5]  
BOOTH C., 1971, The genus Fusarium.
[6]   Chromosomal location and genetic relationship of leaf rust resistance genes Rph9 and Rph12 in barley [J].
Borovkova, IG ;
Jin, Y ;
Steffenson, BJ .
PHYTOPATHOLOGY, 1998, 88 (01) :76-80
[7]   Sexual recombination in Gibberella zeae [J].
Bowden, RL ;
Leslie, JF .
PHYTOPATHOLOGY, 1999, 89 (02) :182-188
[8]   MACROCONIDIUM FORMATION IN SUBMERGED CULTURES BY A NON-SPORULATING STRAIN OF GIBBERELLA ZEAE [J].
CAPPELLINI, RA ;
PETERSON, JL .
MYCOLOGIA, 1965, 57 (06) :962-+
[9]  
Carroll A.M., 1994, FUNGAL GENET NEWSL, V41, P22, DOI [DOI 10.4148/1941-4765.1367, 10.4148/19414765.1367, DOI 10.4148/19414765.1367, 10.4148/1941-4765.1367]
[10]   Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae [J].
Cyert, MS .
ANNUAL REVIEW OF GENETICS, 2001, 35 :647-672