Isolation and sequencing of infectious clones of feline foamy virus and a human/feline foamy virus Env chimera

被引：11

作者：

Hatama, S

Otake, K

Omoto, S

Murase, Y

Ikemoto, A

Mochizuki, M

Takahashi, E

Okuyama, H

Fujii, Y ^{[1
]}

机构：

[1] Nagoya City Univ, Fac Pharmaceut Sci, Dept Biol Chem, Nagoya, Aichi 4678603, Japan

[2] Natl Inst Infect Dis, Dept Immunol, Tokyo 1138657, Japan

[3] Univ Tokyo, Dept Vet Microbiol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[4] Kyoritu Shouji Co Ltd, Cent Lab, Ibaraki, Osaka, Japan

来源：

JOURNAL OF GENERAL VIROLOGY | 2001年 / 82卷

关键词：

D O I：

10.1099/0022-1317-82-12-2999

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Full-length DNAs of the Coleman and S7801 strains (pSKY3.0, pSKY5.0) of infectious feline foamy viruses (FFVs) were cloned and sequenced. Parental viruses, designated SKY3.0 and SKY5.0, were secreted following transfection of Crandell feline kidney (CRFK) cells. Production of the rescued parental viruses was enhanced in the presence of trichostatin A. Amino acid sequence similarities between FFV and human foamy virus (HFV) are extremely low for the envelope protein and capsid antigen, as predicted from the two clones. However, a chimeric FFV clone was constructed with the HFV Env substituted for the FFV Env. The chimeric virus (HFFV, SKY4.0) was able to infect and replicate in CRFK cells as well as in peripheral blood mononuclear cells of cats in vivo. Consequently, the chimeric HFFV may be useful for the creation of FV vectors for gene transfer strategies.

引用

页码：2999 / 3004

页数：6

共 16 条

[1] Functional analysis of human spuma retrovirus genome [J].