Study of DNA-emodin interaction by FTIR and UV-vis spectroscopy

被引：84

作者：

Saito, Samuel T. ^{[1
]}

Silva, Givaldo ^{[1
]}

Pungartnik, Cristina ^{[1
]}

Brendel, Martin ^{[1
]}

机构：

[1] Univ Estadual Santa Cruz, Ctr Biotecnol & Genet, Lab Biol Fungos, Ilheus, BA, Brazil

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2012年 / 111卷

关键词：

Anthraquinone; DNA; Intercalation; Binding constant; Binding mode; ETHIDIUM-BROMIDE; ANTHRAQUINONE; BINDING; PEPTIDES; IR;

D O I：

10.1016/j.jphotobiol.2012.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Emodin, a plant- and fungus-derived anthraquinone, exerts genotoxic and antioxidative effects and shows promise in antitumor and antibacterial therapies. The aim of this study was to examine the molecular interactions of emodin with DNA in aqueous solution at physiological pH using spectroscopic methods. Fourier Transform Infrared (FIR) Spectroscopy and UV absorption spectra were used to determine the structural features, the binding mode and the association constants. Our UV-spectroscopic results indicate that emodin interacts with DNA by intercalation and by external binding. FTIR results suggest that emodin interaction occurs preferably via adenine and thymine base pairs and also weakly with the phosphate backbone of the DNA double helix. The binding constant for emodin-DNA complex formation is estimated to be K = 5.59 x 10(3) M-1. No significant changes of DNA conformation were observed upon emodin-DNA complexation. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：59 / 63

页数：5

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