Biosensor Based on Ultrasmall MoS2 Nanoparticles for Electrochemical Detection of H2O2 Released by Cells at the Nanomolar Level

被引：440

作者：

Wang, Tanyuan ^{[1
]}

Zhu, Haichuan ^{[2
]}

Zhuo, Junqiao ^{[1
]}

Zhu, Zhiwei ^{[1
]}

Papakonstantinou, Pagona ^{[3
]}

Lubarsky, Gennady ^{[3
]}

Lin, Jian ^{[2
]}

Li, Meixian ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, Beijing 100871, Peoples R China

[2] Peking Univ, Coll Chem & Mol Engn, Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China

[3] Univ Ulster, Sch Engn, Engn Res Inst, Newtownabbey BT37 0QB, North Ireland

来源：

ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 21期

基金：

中国国家自然科学基金;

关键词：

HYDROGEN EVOLUTION REACTION; GLUCOSE BIOSENSORS; MODIFIED ELECTRODES; EXTRACELLULAR H2O2; FLUORESCENT-PROBES; CARBON NANOTUBES; GRAPHENE OXIDE; PEROXIDE; SIZE; EXFOLIATION;

D O I：

10.1021/ac402114c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Monodispersed surfactant-free MoS2 nanoparticles with sizes of less than 2 nm were prepared from bulk MoS2 by simple ultrasonication and gradient centrifugation. The ultrasmall MoS2 nanoparticles expose a large fraction of edge sites, along with their high surface area, which lead to attractive electrocatalytic activity for reduction of H2O2. An extremely sensitive H2O2 biosensor based on MoS2 nanoparticles with a real determination limit as low as 2.5 nM and wide linear range of 5 orders of magnitude was constructed. On the basis of this biosensor, the trace amount of H2O2 released from Raw 264.7 cells was successfully recorded, and an efficient glucose biosensor was also fabricated. Since H2O2 is a byproduct of many oxidative biological reactions, this work serves as a pathway for the application of MoS2 in the fields of electrochemical sensing and bioanalysis.

引用

页码：10289 / 10295

页数：7

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