Neospora caninum NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams

被引：12

作者：

Bacigalupe, D. ^{[1
]}

Basso, W. ^{[2
]}

Caspe, S. G. ^{[3
]}

More, G. ^{[1
,5
]}

Lischinsky, L. ^{[4
]}

Gos, M. L. ^{[1
]}

Leunda, M. ^{[4
]}

Campero, L. ^{[1
,5
]}

Moore, D. P. ^{[4
,5
]}

Schares, G. ^{[6
]}

Campero, C. M. ^{[4
]}

Venturini, M. C. ^{[1
]}

机构：

[1] Natl Univ La Plata, Fac Ciencias Vet, Lab Inmunoparasitol, RA-1900 La Plata, Argentina

[2] Univ Zurich, Inst Parasitol, Vetsuisse Fac, CH-8057 Zurich, Switzerland

[3] INTA, Mercedes, Argentina

[4] INTA, Balcarce, Argentina

[5] Consejo Nacl Ciencia & Technol, CONICET, Buenos Aires, DF, Argentina

[6] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, D-16868 Wusterhausen, Germany

来源：

PARASITOLOGY RESEARCH | 2013年 / 112卷 / 07期

关键词：

DAIRY-CATTLE; BOVINE NEOSPOROSIS; DEFINITIVE HOSTS; FETAL-DEATH; INFECTION; DIAGNOSIS; ABORTION; TRANSMISSION; COWS; DIVERSITY;

D O I：

10.1007/s00436-013-3424-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to N. caninum were intravenously inoculated with tachyzoites of the NC-6 Argentina N. caninum strain and slaughtered at 108 +/- 2 days of gestation. Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (gamma IFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for N. caninum. Inoculated animals had significantly higher N. caninum antibody titers and gamma IFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the N. caninum DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.

引用

页码：2585 / 2592

页数：8

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