Development of bovine embryos derived from reproductive techniques

被引:24
作者
Alberto, Miryan L. V. [1 ]
Meirelles, Flavio V. [2 ]
Perecin, Felipe [2 ]
Ambrosio, Carlos E. [2 ]
Favaron, Phelipe O. [1 ]
Franciolli, Andre L. R. [1 ]
Mess, Andrea M. [1 ]
dos Santos, Jose M. [3 ,4 ]
Rici, Rose E. G. [1 ]
Bertolini, Marcelo [5 ]
Miglino, Maria A. [1 ]
机构
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508270 Butanta, SP, Brazil
[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, BR-13635900 Pirassununga, SP, Brazil
[3] Anhembi Morumbi Univ, BR-03164000 Sao Paulo, Brazil
[4] Sch Med ABC, BR-03164000 Sao Paulo, Brazil
[5] Univ Fortaleza, BR-60811905 Fortaleza, Ceara, Brazil
基金
巴西圣保罗研究基金会;
关键词
amnion; animal cloning; chorioallantoic membrane; fixed-time artificial insemination; heart development; IVF; lung development; nuclear transfer; yolk-sac; CELL NUCLEAR TRANSFER; CLONED CATTLE; POSTTRANSFER SURVIVAL; EARLY-PREGNANCY; IN-VIVO; CALVES; FETUSES; FETAL; SHEEP; ASSOCIATION;
D O I
10.1071/RD12092
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Assisted reproduction techniques have improved agricultural breeding in the bovine. However, important development steps may differ from the situation in vivo and there is a high mortality rate during the first trimester of gestation. To better understand these events, we investigated the development of embryos and fetal membranes following fixed-time AI (FTAI), IVF and nuclear transfer (NT). The onset of yolk-sac development was not normal in cloned embryos. Later steps differed from conditions in vivo in all three groups; the yolk-sac was yellowish and juxtaposed with the amniotic membrane. Vascularisation of the chorioallantoic membrane was relatively late and low in NT gestations, but normal in the others. The overall development of the embryos was normal, as indicated by morphology and regression analysis of growth rate. However, NT conceptuses were significantly smaller, with the livers in some embryos occupying the abdominal cavity and others exhibiting heart abnormalities. In conclusion, the yolk-sac and the cardiovascular system seem to be vulnerable to morphogenetic alterations. Future studies will focus on gene expression and early vascularisation processes to investigate whether these changes may be responsible for the high incidence of intrauterine mortality, especially in clones.
引用
收藏
页码:907 / 917
页数:11
相关论文
共 63 条
[1]   Heart fields: one, two or more? [J].
Abu-Issa, R ;
Waldo, K ;
Kirby, ML .
DEVELOPMENTAL BIOLOGY, 2004, 272 (02) :281-285
[2]  
Alves D. F., 2003, Brazilian Journal of Veterinary Research and Animal Science, V40, P279
[3]   Morpho-physical Recording of Bovine Conceptus (Bos indicus) and Placenta from Days 20 to 70 of Pregnancy [J].
Assis Neto, A. C. ;
Pereira, F. T. V. ;
Santos, T. C. ;
Ambrosio, C. E. ;
Leiser, R. ;
Miglino, M. A. .
REPRODUCTION IN DOMESTIC ANIMALS, 2010, 45 (05) :760-772
[4]  
Baruselli P. S., 2003, Brazilian Journal of Veterinary Research and Animal Science, V40, P96
[5]   Perinatal physiology in cloned and normal calves: Physical and clinical characteristics [J].
Batchelder, Cynthia A. ;
Bertolini, Marcelo ;
Mason, Jeffrey B. ;
Moyer, Alice L. ;
Hoffert, Kara A. ;
Petkov, Stoyan G. ;
Famula, Thomas R. ;
Angelos, John ;
George, Lisle W. ;
Anderson, Gary B. .
CLONING AND STEM CELLS, 2007, 9 (01) :63-82
[6]   BIRTH OF LARGE CALVES THAT DEVELOPED FROM IN VITRO-DERIVED BOVINE EMBRYOS [J].
BEHBOODI, E ;
ANDERSON, GB ;
BONDURANT, RH ;
CARGILL, SL ;
KREUSCHER, BR ;
MEDRANO, JF ;
MURRAY, JD .
THERIOGENOLOGY, 1995, 44 (02) :227-232
[7]   Evidence of increased substrate availability to in vitro-derived bovine foetuses and association with accelerated conceptus growth [J].
Bertolini, M ;
Moyer, AL ;
Mason, JB ;
Batchelder, CA ;
Hoffert, KA ;
Bertolini, LR ;
Carneiro, GF ;
Cargill, SL ;
Famula, TR ;
Calvert, CC ;
Sainz, RD ;
Anderson, GB .
REPRODUCTION, 2004, 128 (03) :341-354
[8]   Morphology and morphometry of in vivo- and in vitro-produced bovine concepti from early pregnancy to term and association with high birth weights [J].
Bertolini, M ;
Mason, JB ;
Beam, SW ;
Carneiro, GF ;
Sween, ML ;
Kominek, DJ ;
Moyer, AL ;
Famula, TR ;
Sainz, RD ;
Anderson, GB .
THERIOGENOLOGY, 2002, 58 (05) :973-994
[9]   The placenta as a contributor to production of large calves [J].
Bertolini, M ;
Anderson, GB .
THERIOGENOLOGY, 2002, 57 (01) :181-187
[10]   A history of farm animal embryo transfer and some associated techniques [J].
Betteridge, KJ .
ANIMAL REPRODUCTION SCIENCE, 2003, 79 (3-4) :203-244