Chemical Cytometry: Fluorescence-Based Single-Cell Analysis

被引：95

作者：

Cohen, Daniella ^{[1
]}

Dickerson, Jane A. ^{[1
]}

Whitmore, Colin D. ^{[1
]}

Turner, Emily H. ^{[1
]}

Palcic, Monica M. ^{[2
]}

Hindsgaul, Ole ^{[2
]}

Dovichi, Norman J. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Carlsberg Lab, DK-2500 Valby, Denmark

来源：

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY | 2008年 / 1卷 / 01期

关键词：

fluorescence; capillary electrophoresis; single-cell analysis; cytometry;

D O I：

10.1146/annurev.anchem.1.031207.113104

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ, antibody-based stains to characterize a handful of components in single cells. Chemical cytometry in contrast, employs a suite of powerful analytical tools to characterize a large number of components. Tools have been developed to characterize nucleic acids, proteins, and metabolites in single cells. Whereas nucleic acid analysis employs powerful polymerase chain reaction-based amplification techniques, protein and metabolite analysis tends to employ capillary electrophoresis separation and ultrasensitive laser-induced fluorescence detection. It is now possible to detect yoctomole amounts of many analytes in single cells.

引用

页码：165 / 190

页数：26

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