ORF3 of duck circovirus: A novel protein with apoptotic activity

被引：45

作者：

Xiang, Qi-Wang ^{[1
,2
]}

Wang, Xin ^{[1
,2
]}

Xie, Zhi-Jing ^{[1
,2
]}

Sun, Ya-Ni ^{[3
]}

Zhu, Yan-Li ^{[1
,2
]}

Wang, Shu-Jing ^{[1
,2
]}

Liu, Hong-Jie ^{[1
,2
]}

Jiang, Shi-Jin ^{[1
,2
]}

机构：

[1] Shandong Agr Univ, Dept Vet Prevent Med, Coll Vet Med, Tai An 271018, Shandong, Peoples R China

[2] Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

[3] NW A&F Univ, Coll Vet Med, Yangling 712100, Shanxi, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2012年 / 159卷 / 1-2期

关键词：

Duck circovirus (DuCV); ORF3; IFA; Western blot analysis; Apoptosis; WASTING SYNDROME PMWS; PORCINE; INFECTION; TYPE-2; CHINA; PCR;

D O I：

10.1016/j.vetmic.2012.03.045

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Duck circovirus (DuCV) is classified in the genus Circovirus of the Circoviridae family. Two major open reading frames (ORFs), encoding the replicase (ORF1/rep) and the capsid protein (ORF2/cap), have been recognized for DuCV. Sequence analysis show that another major conserved ORF (named ORB) is located in the complementary strand of ORF1/rep of DuCV, and its function remains to be investigated. In this study, the ORF3 of DuCV was expressed in recombinant baculovirus-infected Sf9 cells. By IFA and Western blot analysis, the ORF3 protein was positive for the sera from ducks infected with DuCV. The percentages of apoptotic cells of the Sf9 cells infected with the recombinant baculovirus encoding ORF3 of DuCV were significantly higher than (P<0.05) that of the Sf9 cells infected with wildtype baculovirus at 24, 48 and 72 h postinfection. Based on our knowledge, we deduced that the ORF3 protein of DuCV might play an important role in viral pathogenesis via its apoptotic activity. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：251 / 256

页数：6

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