Syngas fermentation to biofuels: Effects of hydrogen partial pressure on hydrogenase efficiency

Producing biofuels from gasified biomass (synthesis gas) via microbial fermentation is currently being pursued as one alternative in biofuels development. In synthesis gas fermentation, reducing equivalents from H-2 oxidation via hydrogenase is important towards directing more carbon towards product formation. In this work, kinetic studies of H-2 utilization via the Clostridium P11 hydrogenase enzyme were performed to determine the most appropriate model to predict hydrogenase activity as a function of H-2 partial pressure. An important aspect of this work included the proper analysis of electron acceptors used in the kinetic studies. The K-H2 model parameter governing the effect of H-2 partial pressure on activity was similar to 30 kPa (absolute), independent of the type and concentration of electron acceptor. The K-H2 value indicates that H-2 partial pressures typically associated with syngas fermentation will result in compromised efficiency of the hydrogenase activity. (C) 2013 Elsevier Ltd. All rights reserved.