Guanine oxidation by electron transfer:: One- versus two-electron oxidation mechanism

被引：30

作者：

Kupan, A ^{[1
]}

Saulière, A ^{[1
]}

Broussy, S ^{[1
]}

Seguy, C ^{[1
]}

Pratviel, G ^{[1
]}

Meunier, B ^{[1
]}

机构：

[1] CNRS, Chim Coordinat Lab, F-31077 Toulouse, France

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 01期

关键词：

electron transfer; guanine; porphyrinoids; radical ions; riboflavin;

D O I：

10.1002/cbic.200500284

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The degeneracy of the guanine radical cation, which is formed in DNA by oxidation of guanine by electron transfer, was studied by a detailed analysis of the oxidation products of guanine on oligonucleotide duplexes and by labeling experiments. It was shown that imidazolone, the major product of guanine oxidation, is formed through a one-electron oxidation process and incorporates one oxygen atom from 0, The formation of 8-oxo-7 8-dihydroguanine by a two-electron oxidation process was a minor pathway. The two-electron oxidation mechanism was also evidenced by the formation of a tris(hydroxymethyl)aminomethane adduct.

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页码：125 / 133

页数：9

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