An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based downstream applications

被引：37
|
作者：
Devi, Khumallambam Devala [1 ]
Punyarani, Kshetrimayum [1 ]
Singh, Nandeibam Samarjit [1 ]
Devi, Huidrom Sunitibala [1 ]
机构：
[1] Inst Bioresources & Sustainable Dev, Med Plants & Hort Resources Div, Takyelpat Inst Area, Imphal 795001, Manipur, India
来源：
SPRINGERPLUS | 2013年 / 2卷
关键词：
CTAB; DNA extraction; DNA barcoding gene; ISSR; RAPD; Virus detection; Zingiberales; QUALITY GENOMIC DNA; HERBARIUM SPECIMENS; VIRUS; DISEASE; PLANTS; L;
D O I：
10.1186/2193-1801-2-669
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses.
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页码：1 / 9
页数：9
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