Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection

被引：23
作者：
Chandan, Ravindra K. [1 ,2 ]
Singh, Achuit K. [3 ]
Patel, Sunita [1 ]
Swain, Durga Madhab [2 ]
Tuteja, Narendra [4 ]
Jha, Gopaljee [2 ]
机构：
[1] Cent Univ Gujarat, Sch Life Sci, Gandhinagar, Gujarat, India
[2] Natl Inst Plant Genome Res, Plant Microbe Interact Lab, New Delhi, India
[3] Indian Inst Vegetable Res, Div Crop Improvement, Varanasi, Uttar Pradesh, India
[4] Int Ctr Genet Engn & Biotechnol, Plant Mol Biol Grp, New Delhi, India
来源：
PLANT SIGNALING & BEHAVIOR | 2019年 / 14卷 / 03期
关键词：
LeCTR1; gene; VIGS; marker defence gene; tomato; Tomato leaf curl virus; CAULIFLOWER-MOSAIC-VIRUS; NEGATIVE REGULATOR; SIGNALING PATHWAYS; ARABIDOPSIS; DEFENSE; RESISTANCE; GENE; EVOLUTION; ASSOCIATION; PATHOGEN;
D O I：
10.1080/15592324.2019.1565595
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tomato leaf curl virus (ToLCV) belonging to Begomovirus family of Geminivirus is known to cause one of the most destructive diseases in tomato. Amongst various ToLCVs, a monopartite Tomato leaf curl Joydebpur virus (ToLCJoV) is most prevalent in eastern part of India. In the present study, we observed induced expression of one of the negative regulators of ethylene signaling pathway gene (LeCTR1) in ToLCJoV infected plants. The Tobacco rattle virus (TRV) induced silencing of the LeCTR1 gene provided enhanced tolerance to ToLCJoV infections. The leaf curling as well as ROS accumulation was significantly reduced in the viral infected LeCTR1 silenced plants. Induction of several defense marker genes (NPR1, PR1, PR5, AOS2, EIN2, EIN3 and ERF5) reinforced enhanced tolerance against ToLCJoV infection in the LeCTR1 silenced tomato. Overall, the present study provides evidence that silencing of LeCTR1 can be deployed to protect tomato from ToLCJoV infections.
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页码：1 / 7
页数：7
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