Effect of mulberry leaf (Folium Mori) on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus rats

被引：134
作者：
Cai, Shengyu [1 ]
Sun, Wen [2 ,3 ,4 ]
Fan, Yixin [5 ]
Guo, Xuan [6 ]
Xu, Guangyuan [6 ]
Xu, Tunhai [7 ]
Hou, Yi [6 ]
Zhao, Baosheng [8 ]
Feng, Xingzhong [9 ]
Liu, Tonghua [2 ,3 ,4 ]
机构：
[1] Beijing Univ Chinese Med, Beijing, Peoples R China
[2] Beijing Univ Chinese Med, Key Lab Hlth Cultivat, Minist Educ, Beijing 100029, Peoples R China
[3] Beijing Key Lab Hlth Cultivat, Beijing, Peoples R China
[4] Beijing Int Technol Cooperat Base Prevent & Treat, Beijing, Peoples R China
[5] Beijing Univ Chinese Med, Dept Sci & Technol, Beijing, Peoples R China
[6] Beijing Univ Chinese Med, Dongfang Hosp, Beijing, Peoples R China
[7] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing, Peoples R China
[8] Beijing Univ Chinese Med, Sci Res Expt Ctr, Beijing, Peoples R China
[9] Capital Med Univ, Beijing Shijitan Hosp, Beijing 100038, Peoples R China
来源：
PHARMACEUTICAL BIOLOGY | 2016年 / 54卷 / 11期
关键词：
Antihyperglycemic effect; antihyperlipidemic effect; flavonoids; polyphenols; skeletal muscle; HIGH-FAT DIET; SKELETAL-MUSCLE; OXIDATIVE STRESS; GLUCOSE; DISEASE; EXPRESSION; MICE; ATHEROSCLEROSIS; CHROMATOGRAPHY; POPULATION;
D O I：
10.1080/13880209.2016.1178779
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Context:Folium Mori, the leaf of Morus alba L. (Moraceae), has been used in traditional Chinese medicine (TCM) for treating diabetes. However, it is unclear which components in the mulberry leaf are effective for the treatment of type 2 diabetes mellitus (T2DM).Objective: To investigate the flavonoids and polyphenols in mulberry leaves and their antihyperglycemic and antihyperlipidemic effects in T2DM rats.Materials and methods: Male Sprague-Dawley rats were divided into five groups: normal control (NC), diabetic control (DBC), diabetic group with 0.3mg/kg b.w./day rosiglitazone (RSG), diabetic group with 7g/kg b.w./day TCM formula and diabetic group with 2g/kg b.w./day Folium Mori extract (FME). After 4 weeks, the rats were sacrificed; biochemical parameters, gene and protein expression were measured.Results: The FBG level was significantly lower in the FME group than in the DBC group (p<0.05). In oral glucose tolerance test, the AUC was significantly lower in the FME group (p<0.05). The HOMA-IR level was significantly decreased in the FME group (p<0.05). FME decreased the total cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL) levels (p<0.05). FME increased the mRNA and protein expression of IRS-1, PI3K p85 and Glut-4 increased significantly (p<0.05). Histological analysis revealed amelioration of lipid accumulation following FME treatment. Additionally, immunohistochemical analysis displayed stronger staining of Glut-4 in the FME group compared to the DBC group.Discussion and conclusion: FME could decrease the body weight, blood glucose, TG, TC and LDL levels, and improve insulin resistance. FME possessed significant antihyperglycemic and antihyperlipidemic activities via the IRS-1/PI3K/Glut-4 signalling pathway.
引用
收藏
页码：2685 / 2691
页数：7
相关论文
共 31 条
[1]  
Bharani SER, 2010, PAK J PHARM SCI, V23, P63
[2]   Molecular aspects of glucose homeostasis in skeletal muscle - A focus on the molecular mechanisms of insulin resistance [J].
Carnagarin, Revathy ;
Dharmarajan, Arun M. ;
Dass, Crispin R. .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2015, 417 (0C) :52-62
[3]   Mulberry Leaf Extract Inhibits the Development of Atherosclerosis in Cholesterol-Fed Rabbits and in Cultured Aortic Vascular Smooth Muscle Cells [J].
Chan, Kuei-Chuan ;
Yang, Mon-Yuan ;
Lin, Ming-Cheng ;
Lee, Yi-Ju ;
Chang, Wen-Chun ;
Wang, Chau-Jong .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (11) :2780-2788
[4]   Temporal Trends in the Population Attributable Risk for Cardiovascular Disease The Atherosclerosis Risk in Communities Study [J].
Cheng, Susan ;
Claggett, Brian ;
Correia, Andrew W. ;
Shah, Amil M. ;
Gupta, Deepak K. ;
Skali, Hicham ;
Ni, Hanyu ;
Rosamond, Wayne D. ;
Heiss, Gerardo ;
Folsom, Aaron R. ;
Coresh, Josef ;
Solomon, Scott D. .
CIRCULATION, 2014, 130 (10) :820-828
[5]   Myocardial glucose transport and utilization in patients with type 2 diabetes mellitus, left ventricular dysfunction, and coronary artery disease [J].
Dutka, David P. ;
Pitt, Michael ;
Pagano, Domenico ;
Mongillo, Marco ;
Gathercole, David ;
Bonser, Robert S. ;
Camici, Paolo G. .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2006, 48 (11) :2225-2231
[6]   Rosmarinic acid modulates the antioxidant status and protects pancreatic tissues from glucolipotoxicity mediated oxidative stress in high-fat diet: streptozotocin-induced diabetic rats. [J].
Govindaraj, Jayanthy ;
Pillai, Subramanian Sorimuthu .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2015, 404 (1-2) :143-159
[7]   Fucosylated chondroitin sulfate from Acaudina molpadioides improves hyperglycemia via activation of PKB/GLUT4 signaling in skeletal muscle of insulin resistant mice [J].
Hu, Shiwei ;
Chang, Yaoguang ;
Wang, Jingfeng ;
Xue, Changhu ;
Shi, Di ;
Xu, Hui ;
Wang, Yuming .
FOOD & FUNCTION, 2013, 4 (11) :1639-1646
[8]   Regulation of glucose transporter type 4 isoform gene expression in muscle and adipocytes [J].
Im, Seung-Soon ;
Kwon, Sool-Ki ;
Kim, Tae-Hyun ;
Kim, Ha-il ;
Ahn, Yong-Ho .
IUBMB LIFE, 2007, 59 (03) :134-145
[9]   Functional Role of Serotonin in Insulin Secretion in a Diet-Induced Insulin-Resistant State [J].
Kim, Kyuho ;
Oh, Chang-Myung ;
Ohara-Imaizumi, Mica ;
Park, Sangkyu ;
Namkung, Jun ;
Yadav, Vijay K. ;
Tamarina, Natalia A. ;
Roe, Michael W. ;
Philipson, Louis H. ;
Karsenty, Gerard ;
Nagamatsu, Shinya ;
German, Michael S. ;
Kim, Hail .
ENDOCRINOLOGY, 2015, 156 (02) :444-452
[10]   Paraoxonase1 (PON1) reduces insulin resistance in mice fed a high-fat diet, and promotes GLUT4 overexpression in myocytes, via the IRS-1/Akt pathway [J].
Koren-Gluzer, Marie ;
Aviram, Michael ;
Hayek, Tony .
ATHEROSCLEROSIS, 2013, 229 (01) :71-78
1234