A simple method for the quantitative analysis of resin bound thiol groups

被引:55
作者
Badyal, JP
Cameron, AM
Cameron, NR
Coe, DM
Cox, R
Davis, BG
Oates, LJ
Oye, G
Steel, PG
机构
[1] Univ Durham, Dept Chem, Sci Labs, Durham DH1 3LE, England
[2] GlaxoSmithKline, Med Res Ctr, Stevenage SG1 2NY, Herts, England
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1016/S0040-4039(01)01820-2
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
Non-aqueous solutions of Ellman's reagent [5,5'-dithio(2-nitrobenzoic acid), DTNB] can be used to quantify thiols supported on macroporous polystyrene and TentaGel resins. Organic solutions of Ellman's reagent may also be used as a qualitative tests for thiols on a wider range of solid supports. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:8531 / 8533
页数:3
相关论文
共 31 条
  • [1] Malachite green, a valuable reagent to monitor the presence of free COOH on the solid-phase
    Attardi, ME
    Porcu, G
    Taddei, M
    [J]. TETRAHEDRON LETTERS, 2000, 41 (38) : 7391 - 7394
  • [2] Polymers with thiol groups:: A new generation of mucoadhesive polymers?
    Bernkop-Schnürch, A
    Schwarz, V
    Steininger, S
    [J]. PHARMACEUTICAL RESEARCH, 1999, 16 (06) : 876 - 881
  • [3] NPIT - A NEW REAGENT FOR QUANTITATIVELY MONITORING REACTIONS OF AMINES IN COMBINATORIAL SYNTHESIS
    CHU, SS
    REICH, SH
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1995, 5 (10) : 1053 - 1058
  • [4] Glycosyldisulfides: a new class of solution and solid phase glycosyl donors
    Davis, BG
    Ward, SJ
    Rendle, PM
    [J]. CHEMICAL COMMUNICATIONS, 2001, (02) : 189 - 190
  • [5] de Miguel YR, 2000, BIOTECHNOL BIOENG, V71, P119, DOI 10.1002/1097-0290(2000)71:2<119::AID-BIT1003>3.0.CO
  • [6] 2-R
  • [7] A method for quantitation of solid-phase synthesis using 19F NMR spectroscopy
    Drew, M
    Orton, E
    Krolikowski, P
    Salvino, JM
    Kumar, NV
    [J]. JOURNAL OF COMBINATORIAL CHEMISTRY, 2000, 2 (01): : 8 - 9
  • [8] Ellman G. L., 1972, J AM CHEM SOC, V94, P3102
  • [9] TISSUE SULFHYDRYL GROUPS
    ELLMAN, GL
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) : 70 - 77
  • [10] Fang LL, 2000, BIOTECHNOL BIOENG, V71, P162, DOI 10.1002/1097-0290(2000)71:2<162::AID-BIT1006>3.0.CO