Identification and Characterization of Eimeria tenella Apical Membrane Antigen-1 (AMA1)

被引：63

作者：

Jiang, Lianlian ^{[1
]}

Lin, Jiaojiao ^{[1
]}

Han, Hongyu ^{[1
]}

Dong, Hui ^{[1
]}

Zhao, Qiping ^{[1
]}

Zhu, Shunhai ^{[1
]}

Huang, Bing ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Anim Parasitol, Minist Agr, Shanghai Vet Res Inst, Shanghai, Peoples R China

来源：

PLOS ONE | 2012年 / 7卷 / 07期

关键词：

MALARIA VACCINE CANDIDATE; TOXOPLASMA-GONDII; HOST-CELL; INVASION; COCCIDIOSIS; INFECTION; PARASITES; PURIFICATION; MEROZOITES; EXPRESSION;

D O I：

10.1371/journal.pone.0041115

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends technique. EtAMA1 had an open reading frame of 1608 bp encoding a protein of 535 amino acids. Quantitative real-time PCR analysis revealed that EtAMA1 was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts and second-generation merozoites). The ectodomain sequence was expressed as recombinant EtAMA1 (rEtAMA1) and rabbit polyclonal antibodies raised against the rEtAMA1 recognized a 58-kDa native parasite protein by Western Blotting and had a potent inhibitory effect on parasite invasion, decreasing it by approximately 70%. Immunofluorescence analysis and immunohistochemistry analysis showed EtAMA1 might play an important role in sporozoite invasion and development.

引用

页数：9

共 43 条

[1] Identification of the moving junction complex of Toxoplasma gondii:: A collaboration between distinct secretory organelles [J].