Screening of yeast with high-yield Single Cell Protein and preliminary optimization of its culture codition

被引:0
|
作者
Shi, Min [1 ]
Liang, Mingzheng [1 ]
Liang, Hongyu [1 ]
Li, Mei [1 ]
Liao, Anping [1 ]
机构
[1] Guangxi Univ, Guangxi Univ Nationalities, Coll Chem & Ecoengn, Key Lab Chem & Biol Transforming Proc, Nanning 530006, Peoples R China
来源
ADVANCED MATERIALS, PTS 1-3 | 2012年 / 415-417卷
关键词
Coomassie brilliant blue methods; SCP; Candida tropicalis; Optimization; cultivating condition;
D O I
10.4028/www.scientific.net/AMR.415-417.1561
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The protein content of Rhodotorula rubra 2.1817,Candida tropicalis 2.1775 and Geotrichum candidum 2.1175 were assayed by Coomassie brilliant blue methods. Screening the high-yield SCP strains. Then,the cultur condition of the strains were optimizated by single-factor test. It is shown that the high-yield SCP strains was Candida tropicalis 2.1775,the protein content was 32.46%.The optimal factors: inoculum size 10% (V/V),temperaturc 30 degrees C,shaking 200 rpm,culture time 5 days.
引用
收藏
页码:1561 / 1564
页数:4
相关论文
共 50 条
  • [1] Screening, Identification, and Fermentation Condition Optimization of a High-Yield 3-Methylthiopropanol Yeast and Its Aroma-Producing Characteristics
    Zhang, Yujiao
    Sun, Qi
    Liu, Xiaoyan
    Basit, Rana Abdul
    Ma, Jinghao
    Fu, Zhilei
    Cheng, Liujie
    Fan, Guangsen
    Teng, Chao
    FOODS, 2024, 13 (03)
  • [2] Screening of an Alcohol Tolerant and High-yield L-lactic Acid Strain and Optimization of Culture Medium
    Xiao M.
    Xing X.
    Liu W.
    Meng X.
    Wei S.
    Zhang T.
    Wang Y.
    Li X.
    Science and Technology of Food Industry, 2023, 44 (08) : 135 - 143
  • [3] Isolation and Identification of a High-Yield Ethyl Caproate-Producing Yeast From Daqu and Optimization of Its Fermentation
    Fan, Guangsen
    Liu, Pengxiao
    Chang, Xu
    Yin, Huan
    Cheng, Liujie
    Teng, Chao
    Gong, Yi
    Li, Xiuting
    FRONTIERS IN MICROBIOLOGY, 2021, 12
  • [4] Optimization of cell culture for H9N2 subtype AIV and establishment of high-yield cell strain
    Lu, Yu-Jian
    Wu, Xin-Ming
    Guan, Yu
    Zhang, Song-Lin
    Wang, Jin-Liang
    Zhao, Jiu-Xia
    Han, Wen-Yu
    Shen, Zhi-Qiang
    BULGARIAN CHEMICAL COMMUNICATIONS, 2017, 49 (01): : 161 - 170
  • [5] Screening Strategies for High-Yield Chinese Hamster Ovary Cell Clones
    Yang, Wenwen
    Zhang, Junhe
    Xiao, Yunxi
    Li, Wenqing
    Wang, Tianyun
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 10
  • [6] Optimization of high-yield biological synthesis of single-crystalline gold nanoplates
    Liu, B
    Xie, J
    Lee, JY
    Ting, YP
    Chen, JP
    JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (32): : 15256 - 15263
  • [7] REDUCING THE REQUIREMENT FOR SERUM SUPPLEMENTS IN HIGH-YIELD MICROCARRIER CELL-CULTURE
    CLARK, JM
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1983, 364 (05): : 470 - 470
  • [8] High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris
    Liu, Ru-Shi
    Yang, Kun-Yu
    Lin, Jian
    Lin, Yi-Wei
    Zhang, Zhi-Hong
    Zhang, Jun
    Xia, Ning-Shao
    WORLD JOURNAL OF GASTROENTEROLOGY, 2004, 10 (24) : 3602 - 3607
  • [9] High-yield expression of recombinant SARS coronavirus nudeocapsid protein in methylotrophic yeast Pichia pastoris
    Ru-Shi Liu Kun-Yu Yang Jian Lin Yi-Wei Lin Zhi-Hong Zhang Jun Zhang Ning-Shao Xia The Key Laboratory for Cell Biology and Tumor Cell Engineering of the Ministry of Education
    The Research Center for Medical Molecular Virology of Fujian Province
    World Journal of Gastroenterology, 2004, (24) : 3602 - 3607
  • [10] High-yield single-use cell culture systems - Tutorial: Using disposables to reduce labor and increase yields
    Vyas, R
    Cino, J
    GENETIC ENGINEERING NEWS, 2005, 25 (13): : 48 - 49