Quantitative 3D investigation of Neuronal network in mouse spinal cord model

被引：36

作者：

Bukreeva, I. ^{[1
]}

Campi, G. ^{[2
]}

Fratini, M. ^{[1
,3
]}

Spano, R. ^{[4
,5
]}

Bucci, D. ^{[6
]}

Battaglia, G. ^{[6
]}

Giove, F. ^{[7
,8
]}

Bravin, A. ^{[9
]}

Uccelli, A. ^{[10
,11
]}

Venturi, C. ^{[10
]}

Mastrogiacomo, M. ^{[4
,5
]}

Cedola, A. ^{[1
]}

机构：

[1] Sapienza Univ, CNR, Inst Nanotechnol, Dept Phys, Piazzale Aldo Moro 2, I-00185 Rome, Italy

[2] CNR, Inst Crystallog, I-00015 Rome, Italy

[3] Fdn Santa Lucia, IRCCS, Via Ardeatina 306, I-00179 Rome, Italy

[4] Univ Genoa, Dept Expt Med, Largo R Benzi 10, I-16132 Genoa, Italy

[5] AUO San Martino IST Ist Nazl Ric Cancro, Largo R Benzi 10, I-16132 Genoa, Italy

[6] IRCCS, Neuromed, I-86077 Pozzilli, Italy

[7] Museo Storico Fis, Piazza Viminale 1, I-00184 Rome, Italy

[8] Ctr & Ric Enrico Fermi, Piazza Viminale 1, I-00184 Rome, Italy

[9] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38043 Grenoble, France

[10] Univ Genoa, DINOGMI, Largo Daneo 3, IT-16132 Genoa, Italy

[11] Azienda Osped Univ San Martino, IRCCS, IST, Genoa, Italy

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

RETINAL MOSAICS; PHASE RETRIEVAL; ENCEPHALOMYELITIS; ORGANIZATION; STEREOLOGY; SOFTWARE; SYSTEMS;

D O I：

10.1038/srep41054

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The investigation of the neuronal network in mouse spinal cord models represents the basis for the research on neurodegenerative diseases. In this framework, the quantitative analysis of the single elements in different districts is a crucial task. However, conventional 3D imaging techniques do not have enough spatial resolution and contrast to allow for a quantitative investigation of the neuronal network. Exploiting the high coherence and the high flux of synchrotron sources, X-ray Phase-Contrast multiscale-Tomography allows for the 3D investigation of the neuronal microanatomy without any aggressive sample preparation or sectioning. We investigated healthy-mouse neuronal architecture by imaging the 3D distribution of the neuronal-network with a spatial resolution of 640 nm. The high quality of the obtained images enables a quantitative study of the neuronal structure on a subject-by-subject basis. We developed and applied a spatial statistical analysis on the motor neurons to obtain quantitative information on their 3D arrangement in the healthy-mice spinal cord. Then, we compared the obtained results with a mouse model of multiple sclerosis. Our approach paves the way to the creation of a "database" for the characterization of the neuronal network main features for a comparative investigation of neurodegenerative diseases and therapies.

引用

页数：10

共 33 条

[1] A role for caspase-1 and-3 in the pathology of experimental allergic encephalomyelitis -: Inflammation versus degeneration [J].