Synergistic Role of Bacterial Urease and Carbonic Anhydrase in Carbonate Mineralization

被引：144

作者：

Dhami, Navdeep Kaur ^{[1
]}

Reddy, M. S. ^{[1
]}

Mukherjee, Abhijit ^{[2
]}

机构：

[1] Thapar Univ, Dept Biotechnol, Patiala 147004, Punjab, India

[2] Indian Inst Technol, Dept Civil Engn, Gandhinagar 382424, Gujarat, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2014年 / 172卷 / 05期

关键词：

Bacillus megaterium; CaCO3; precipitation; Bacterial urease; Bacterial carbonic anhydrase; Acetohydroxamic acid; Acetazolamide; PRECIPITATION; IMPROVEMENT;

D O I：

10.1007/s12010-013-0694-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The investigation on the synergistic role of urease (UA) and carbonic anhydrase (CA) in biomineralization of calcium carbonate in Bacillus megaterium suggested that the precipitation of CaCO3 is significantly faster in bacterial culture than in crude enzyme solutions. Calcite precipitation is significantly reduced when both the enzymes are inhibited in comparison with those of the individual enzyme inhibitions indicating that both UA and CA are crucial for efficient mineralization. Carbonic anhydrase plays a role in hydrating carbon dioxide to bicarbonate, while UA aids in maintaining the alkaline pH that promotes calcification process.

引用

页码：2552 / 2561

页数：10

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