A Switchable Two-Photon Membrane Tracer Capable of Imaging Membrane-Associated Protein Tyrosine Phosphatase Activities

被引：86
作者：
Li, Lin [1 ]
Shen, Xiaoqin [1 ]
Xu, Qing-Hua [1 ]
Yao, Shao Q. [1 ]
机构：
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 01期
关键词：
bioimaging; fluorescence microscopy; membrane proteins; phosphatases; small-molecule probes; ACTIVITY-BASED PROBES; FLUORESCENT-PROBES; ENZYME-ACTIVITY; INHIBITORS; MECHANISM; CELLS;
D O I：
10.1002/anie.201205940
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
When I look into your cells: A two-photon dye (Flu7) was developed (see scheme), which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV- and phosphatase-controlled quencher (Q12), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane-associated receptor-like protein tyrosine phosphatase (RPTP) activity. © 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：424 / 428
页数：5
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