A Novel Escherichia coli O157:H7 Clone Causing a Major Hemolytic Uremic Syndrome Outbreak in China

被引：65

作者：

Xiong, Yanwen ^{[1
]}

Wang, Ping ^{[1
]}

Lan, Ruiting ^{[2
]}

Ye, Changyun ^{[1
]}

Wang, Hua ^{[3
]}

Ren, Jun ^{[4
]}

Jing, Huaiqi ^{[1
]}

Wang, Yiting ^{[1
]}

Zhou, Zhemin ^{[5
]}

Bai, Xuemei ^{[1
]}

Cui, Zhigang ^{[1
]}

Luo, Xia ^{[1
]}

Zhao, Ailan ^{[1
]}

Wang, Yan ^{[1
]}

Zhang, Shaomin ^{[1
]}

Sun, Hui ^{[1
]}

Wang, Lei ^{[5
]}

Xu, Jianguo ^{[1
]}

机构：

[1] Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China

[2] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia

[3] Jiangsu Prov Ctr Dis Control & Prevent, Nanjing, Jiangsu, Peoples R China

[4] Anhui Prov Ctr Dis Control & Prevent, Hefei, Anhui, Peoples R China

[5] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

来源：

PLOS ONE | 2012年 / 7卷 / 04期

关键词：

SHIGA-TOXIN; HEMORRHAGIC COLITIS; O157-H7; VIRULENCE; SEQUENCE; STRAINS; O157H7; SECRETION; GENOTYPES; PROFILES;

D O I：

10.1371/journal.pone.0036144

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.

引用

页数：10

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