Iron oxide nanoparticles;
Oxidative damage;
Human bone marrow mesenchymal stromal cells;
MAGNETIC-RESONANCE TRACKING;
EMBRYONIC STEM-CELLS;
SPINAL-CORD-INJURY;
IN-VIVO TRACKING;
DNA-DAMAGE;
LIPID-PEROXIDATION;
COMET ASSAY;
INTRACELLULAR UPTAKE;
PROTEIN OXIDATION;
RAT-BRAIN;
D O I:
10.1016/j.toxlet.2012.01.008
中图分类号:
R99 [毒物学(毒理学)];
学科分类号:
100405 ;
摘要:
The biological effects of several superparamagnetic iron oxide nanoparticles (SPIONs) varying in their surface coating were tested using human bone marrow mesenchymal stromal cells from two donors - hBMSCs-1 and hBMSCs-2. The measurements were performed at two intervals - after 72 h exposure to the nanoparticles and after an additional 72 h cell growth without nanoparticles. The dose of SPIONs used (15.4 mu g Fe/ml) was selected as being sufficient for in vivo cell tracking using magnetic resonance imaging (MRI). Concerning cell viability and cell death, only the hBMSCs-2 seemed to be sensitive to the action of SPIONs. However, an increase of oxidative injury to lipids, proteins and DNA as a consequence of exposure to SPIONs was detected in cells from both donors. Particularly the levels of lipid peroxidation were high and increased further with time, regardless of the type of nanoparticle. Lowering intracellular label concentrations and authenticating oxidative stress levels using in vivo experiments are required to ensure the safety of SPIONs for biomedical applications. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Gupta, Ajay Kumar
Naregalkar, Rohan R.
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机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Naregalkar, Rohan R.
Vaidya, Vikas Deep
论文数: 0引用数: 0
h-index: 0
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Vaidya, Vikas Deep
Gupta, Mona
论文数: 0引用数: 0
h-index: 0
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
机构:
Univ Paris 07, Paris, France
CNRS, Paris, France
INSERM, Paris, France
Univ Paris 05, Paris, France
Univ Carthage, Jarzouna, TunisiaUniv Paris 07, Paris, France
Hanini, Amel
Schmitt, Alain
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, Paris, France
INSERM, Paris, France
Univ Paris 05, Paris, FranceUniv Paris 07, Paris, France
Schmitt, Alain
Kacem, Kamel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Carthage, Jarzouna, TunisiaUniv Paris 07, Paris, France
Kacem, Kamel
Chau, Francois
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 07, Paris, France
CNRS, Paris, FranceUniv Paris 07, Paris, France
Chau, Francois
论文数: 引用数:
h-index:
机构:
Ammar, Souad
论文数: 引用数:
h-index:
机构:
Gavard, Julie
[J].
INTERNATIONAL JOURNAL OF NANOMEDICINE,
2011,
6
: 787
-
794
机构:
Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Harper, S.
Usenko, C.
论文数: 0引用数: 0
h-index: 0
机构:
Baylor Univ, Dept Biol, Waco, TX 76798 USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Usenko, C.
Hutchison, J. E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USA
Univ Oregon, Dept Chem, Eugene, OR 97403 USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Hutchison, J. E.
Maddux, B. L. S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Maddux, B. L. S.
Tanguay, R. L.
论文数: 0引用数: 0
h-index: 0
机构:
Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Gupta, Ajay Kumar
Naregalkar, Rohan R.
论文数: 0引用数: 0
h-index: 0
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Naregalkar, Rohan R.
Vaidya, Vikas Deep
论文数: 0引用数: 0
h-index: 0
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
Vaidya, Vikas Deep
Gupta, Mona
论文数: 0引用数: 0
h-index: 0
机构:
TPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, IndiaTPL, Torrent Res Ctr, Formulat & Dev Dept, Lab Nanoparticle Res, Village Bhat 382428, Gujarat, India
机构:
Univ Paris 07, Paris, France
CNRS, Paris, France
INSERM, Paris, France
Univ Paris 05, Paris, France
Univ Carthage, Jarzouna, TunisiaUniv Paris 07, Paris, France
Hanini, Amel
Schmitt, Alain
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, Paris, France
INSERM, Paris, France
Univ Paris 05, Paris, FranceUniv Paris 07, Paris, France
Schmitt, Alain
Kacem, Kamel
论文数: 0引用数: 0
h-index: 0
机构:
Univ Carthage, Jarzouna, TunisiaUniv Paris 07, Paris, France
Kacem, Kamel
Chau, Francois
论文数: 0引用数: 0
h-index: 0
机构:
Univ Paris 07, Paris, France
CNRS, Paris, FranceUniv Paris 07, Paris, France
Chau, Francois
论文数: 引用数:
h-index:
机构:
Ammar, Souad
论文数: 引用数:
h-index:
机构:
Gavard, Julie
[J].
INTERNATIONAL JOURNAL OF NANOMEDICINE,
2011,
6
: 787
-
794
机构:
Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Harper, S.
Usenko, C.
论文数: 0引用数: 0
h-index: 0
机构:
Baylor Univ, Dept Biol, Waco, TX 76798 USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Usenko, C.
Hutchison, J. E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USA
Univ Oregon, Dept Chem, Eugene, OR 97403 USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Hutchison, J. E.
Maddux, B. L. S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Maddux, B. L. S.
Tanguay, R. L.
论文数: 0引用数: 0
h-index: 0
机构:
Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA
Univ Oregon, ONAMI Safer Nanomat & Nanomfg Initiat, Corvallis, OR USAOregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA