Isolation and characterization of exosomes released from mosquito cells infected with dengue virus

被引：65

作者：

Manuel Reyes-Ruiz, Jose ^{[1
]}

Fidel Osuna-Ramos, Juan ^{[1
]}

Adrian De Jesus-Gonzalez, Luis ^{[1
]}

Mahely Hurtado-Monzon, Arianna ^{[1
]}

Noe Farfan-Morales, Carlos ^{[1
]}

Cervantes-Salazar, Margot ^{[1
]}

Bolanos, Jeni ^{[1
]}

Cigarroa-Mayorga, Oscar E. ^{[5
]}

San Martin-Martinez, Eduardo ^{[6
]}

Medina, Fernando ^{[1
]}

Jaime Fragoso-Soriano, Rogelio ^{[4
]}

Chavez-Munguia, Bibiana ^{[1
]}

Santiago Salas-Benito, Juan ^{[2
,3
]}

del Angel, Rosa M. ^{[1
]}

机构：

[1] Ctr Res & Adv Studies CINVESTAV IPN, Dept Infect & Mol Pathogenesis, Mexico City, DF, Mexico

[2] Inst Politecn Nacl, Escuela Nacl Med & Homeopatia, Ciencias Biomed Mol, Mexico City, DF, Mexico

[3] Inst Politecn Nacl, Escuela Nacl Med & Homeopatia, Ciencias Biotecnol, Mexico City, DF, Mexico

[4] Ctr Res & Adv Studies CINVESTAV IPN, Dept Phys, Mexico City, DF, Mexico

[5] Inst Politecn Nacl, Dept Tecnol Avanzadas, Unidad Profes Interdisciplinaria Ingn & Tecnol Av, Mexico City, DF, Mexico

[6] IPN, CICATA, Mexico City, DF, Mexico

来源：

VIRUS RESEARCH | 2019年 / 266卷

关键词：

Dengue virus; Exosomes; Intraluminal vesicles (ILVs); Multivesicular bodies (MVBs); C6/36; cells; HEPATITIS-C VIRUS; 3-DIMENSIONAL ARCHITECTURE; TETRASPANIN SUPERFAMILY; PROTEOMICS ANALYSIS; IN-VITRO; REPLICATION; ASSOCIATION; BIOGENESIS; SECRETION; CD81;

D O I：

10.1016/j.virusres.2019.03.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naive C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.

引用

页码：1 / 14

页数：14

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