Molecular basis of mammalian cell invasion by Trypanosoma cruzi

被引:185
作者
Yoshida, N [1 ]
机构
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
来源
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2006年 / 78卷 / 01期
关键词
Trypanosoma cruzi; trypomastigotes; cell invasion; signal transduction; Ca2+ mobilization;
D O I
10.1590/S0001-37652006000100010
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Establishment of infection by Trypanosoma cruzi, the agent of Chagas' disease, depends on a series of events involving interactions of diverse parasite molecules with host components. Here we focus on the mechanisms of target cell invasion by metacyclic trypomastigotes (NIT) and mammalian tissue culture trypomastigotes (TCT). During NIT or TCT internalization, signal transduction pathways are activated both in the parasite and the target cell, leading to Ca2+ mobilization. For cell adhesion, NIT engage surface glycoproteins, such as gp82 and gp35/50, which are Ca2+ signal-inducing molecules. In T cruzi isolates that enter host cells in gp82-mediated manner, parasite protein tyrosine kinase as well as phospholipase C are activated, and Ca2+ is released from IP3-sensitive stores, whereas in T cruzi isolates that attach to target cells mainly through gp35/50, the signaling pathway involving adenylate cyclase appears to be stimulated, with Ca2+ release from acidocalciosomes. In addition, T cruzi isolate-dependent inhibitory signals, mediated by NIT-specific gp90, may be triggered both in the host cell and the parasite. The repertoire of TCT molecules implicated in cell invasion includes surface glycoproteins of gp85 family, with members containing binding sites for laminin and cytokeratin 18, enzymes such as cruzipain, trans-sialidase, and an oligopeptidase B that generates a Ca2+-agonist from a precursor molecule.
引用
收藏
页码:87 / 111
页数:25
相关论文
共 127 条
  • [1] The mucin-like glycoprotein super-family of Trypanosoma cruzi:: structure and biological roles
    Acosta-Serrano, A
    Almeida, IC
    Freitas, LH
    Yoshida, N
    Schenkman, S
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 114 (02) : 143 - 150
  • [2] Acosta-Serrano A., 1995, J BIOL CHEM, V270, P27244
  • [3] LYTIC ANTI-ALPHA-GALACTOSYL ANTIBODIES FROM PATIENTS WITH CHRONIC CHAGAS-DISEASE RECOGNIZE NOVEL O-LINKED OLIGOSACCHARIDES ON MUCIN-LIKE GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED GLYCOPROTEINS OF TRYPANOSOMA-CRUZI
    ALMEIDA, IC
    FERGUSON, MAJ
    SCHENKMAN, S
    TRAVASSOS, LR
    [J]. BIOCHEMICAL JOURNAL, 1994, 304 : 793 - 802
  • [4] ALVES MJM, 1986, MOL BIOCHEM PARASIT, V21, P75, DOI 10.1016/0166-6851(86)90081-2
  • [5] Lysosomal fusion is essential for the retention of Trypanosoma cruzi inside host cells
    Andrade, LO
    Andrews, NW
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 200 (09) : 1135 - 1143
  • [6] LYSOSOME RECRUITMENT DURING HOST-CELL INVASION BY TRYPANOSOMA-CRUZI
    ANDREWS, NW
    [J]. TRENDS IN CELL BIOLOGY, 1995, 5 (03) : 133 - 137
  • [7] A new cruzipain-mediated pathway of human cell invasion by Trypanosoma cruzi requires trypomastigote membranes
    Aparicio, IM
    Scharfstein, J
    Lima, APCA
    [J]. INFECTION AND IMMUNITY, 2004, 72 (10) : 5892 - 5902
  • [8] CLONING AND CHARACTERIZATION OF A GENE FOR THE STAGE-SPECIFIC 82-KDA SURFACE-ANTIGEN OF METACYCLIC TRYPOMASTIGOTES OF TRYPANOSOMA-CRUZI
    ARAYA, JE
    CANO, MI
    YOSHIDA, N
    DASILVEIRA, JF
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 65 (01) : 161 - 169
  • [9] Molecular basis of non-virulence of Trypanosoma cruzi clone CL-14
    Atayde, VD
    Neira, I
    Cortez, M
    Ferreira, D
    Freymüller, E
    Yoshida, N
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2004, 34 (07) : 851 - 860
  • [10] EVIDENCE OF PARTICIPATION OF CYTOSKELETON OF HEART-MUSCLE CELLS DURING THE INVASION OF TRYPANOSOMA-CRUZI
    BARBOSA, HS
    MEIRELLES, MNL
    [J]. CELL STRUCTURE AND FUNCTION, 1995, 20 (04) : 275 - 284