Characterization of brevetoxin metabolism in Karenia brevis bloom-exposed clams (Mercenaria sp.) by LC-MS/MS

被引：28

作者：

Abraham, Ann ^{[1
]}

Wang, Yuesong ^{[1
]}

El Said, Kathleen R. ^{[1
]}

Plakas, Steven M. ^{[1
]}

机构：

[1] US FDA, Div Seafood Sci & Technol, Gulf Coast Seafood Lab, Dauphin Isl, AL 36528 USA

来源：

TOXICON | 2012年 / 60卷 / 06期

关键词：

Brevetoxins; Karenia brevis; Clams; LC-MS; OYSTER CRASSOSTREA-VIRGINICA; MUSSELS PERNA-CANALICULUS; NEW-ZEALAND; RED TIDE; GREENSHELL MUSSELS; AUSTROVENUS-STUTCHBURYI; CONTROLLED EXPOSURES; PTYCHODISCUS-BREVIS; SHELLFISH; TOXIN;

D O I：

10.1016/j.toxicon.2012.06.016

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Brevetoxin metabolites were identified and characterized in the hard clam (Mercenaria sp.) after natural exposure to Karenia brevis blooms by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Principal brevetoxins BTX-1 and BTX-2 produced by K. brevis were not detectable in clams. Metabolites of these brevetoxins found in clams included products of oxidation, reduction, hydrolysis and amino acid/fatty acid conjugation. Of highest abundance were cysteine and taurine conjugates. We also found glutathione, glycine-cysteine, and gamma-glutamyl-cysteine conjugates. A series of fatty acid derivatives of cysteine-brevetoxin conjugates were also identified. Published by Elsevier Ltd.

引用

页码：1030 / 1040

页数：11

共 19 条

[1] Characterization of polar brevetoxin derivatives isolated from Karenia brevis cultures and natural blooms [J].

Abraham, Ann ;

Plakas, Steven M. ;

Wang, Zhihong ;

Jester, Edward L. E. ;

El Said, Kathleen R. ;

Granade, Hudson R. ;

Henry, Michael S. ;

Blum, Patricia C. ;

Pierce, Richard H. ;

Dickey, Robert W. .

TOXICON, 2006, 48 (01) :104-115

[2] Natural and derivative brevetoxins: Historical background, multiplicity, and effects [J].

Baden, DG ;

Bourdelais, AJ ;

Jacocks, H ;

Michelliza, S ;

Naar, J .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2005, 113 (05) :621-625

[3]

Dickey R, 1999, NAT TOXINS, V7, P157, DOI 10.1002/(SICI)1522-7189(199907/08)7:4<157::AID-NT52>3.3.CO

[4]

2-R

[5] Electrospray ionization tandem mass spectrometry for structural elucidation of protonated brevetoxins in red tide algae [J].

Hua, YS ;

Cole, RB .

ANALYTICAL CHEMISTRY, 2000, 72 (02) :376-383

[6] Brevetoxin B5, a new brevetoxin analog isolated from cockle Austrovenus stutchburyi in New Zealand, the marker for monitoring shellfish neurotoxicity [J].

Ishida, H ;

Nozawa, A ;

Hamano, H ;

Naoki, H ;

Fujita, T ;

Kaspar, HF ;

Tsuji, K .

TETRAHEDRON LETTERS, 2004, 45 (01) :29-33

[7] BREVETOXIN-B-1, A NEW POLYETHER MARINE TOXIN FROM THE NEW-ZEALAND SHELLFISH, AUSTROVENUS-STUTCHBURYI [J].

ISHIDA, H ;

NOZAWA, A ;

TOTORIBE, K ;

MURAMATSU, N ;

NUKAYA, H ;

TSUJI, K ;

YAMAGUCHI, K ;

YASUMOTO, T ;

KASPAR, H ;

BERKETT, N ;

KOSUGE, T .

TETRAHEDRON LETTERS, 1995, 36 (05) :725-728

[8] ISOLATION AND STRUCTURE OF BREVETOXIN-B FROM THE RED TIDE DINOFLAGELLATE PTYCHODISCUS-BREVIS (GYMNODINIUM, BREVE) [J].

LIN, YY ;

RISK, M ;

RAY, SM ;

VANENGEN, D ;

CLARDY, J ;

GOLIK, J ;

JAMES, JC ;

NAKANISHI, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1981, 103 (22) :6773-6775

[9] Complex toxin profiles in phytoplankton and Greenshell mussels (Perna canaliculus), revealed by LC-MS/MS analysis [J].

MacKenzie, L ;

Holland, P ;

McNabb, P ;

Beuzenberg, V ;

Selwood, A ;

Suzuki, T .

TOXICON, 2002, 40 (09) :1321-1330

[10] BREVETOXIN B3, A NEW BREVETOXIN ANALOG ISOLATED FROM THE GREENSHELL MUSSEL PERNA-CANALICULUS INVOLVED IN NEUROTOXIC SHELLFISH POISONING IN NEW-ZEALAND [J].

MOROHASHI, A ;

SATAKE, M ;

MURATA, K ;

NAOKI, H ;

KASPAR, HF ;

YASUMOTO, T .

TETRAHEDRON LETTERS, 1995, 36 (49) :8995-8998

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