Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source

被引：23

作者：

Dai, Cuixia ^{[2
]}

Liu, Xiaojing ^{[1
]}

Jiao, Shuliang ^{[1
]}

机构：

[1] Univ So Calif, Keck Sch Med, Dept Ophthalmol, Los Angeles, CA 90033 USA

[2] Shanghai Inst Technol, Coll Sci, Shanghai 201418, Peoples R China

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 08期

基金：

美国国家卫生研究院;

关键词：

dual-modality imaging; optical coherence tomography; autofluorescence microscopy; FLUORESCENCE SPECTROSCOPY; CANCER; MODEL;

D O I：

10.1117/1.JBO.17.8.080502

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have accomplished simultaneous spectral domain optical coherence tomography (SD-OCT) and autofluorescence (AF) microscopy with a broadband light source centered at 415 nm. The light source was provided by frequency-doubling of an ultra-fast broadband Ti:Sapphire laser. With a bandwidth of 8 nm, the visible SD-OCT achieved a depth resolution of similar to 12 p mu group of photons, their images are intrinsically registered. The dual-modal system is capable of providing OCT imaging and molecular contrasts simultaneously. The imaging system was tested on imaging biological samples ex vivo and in vivo. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOL 10.1117/1.JBO.17.8.080502]

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