Protein-induced fusion of phospholipid vesicles of heterogeneous sizes

被引：22
作者：
de Arcuri, BF
Vechetti, GF
Chehín, RN
Goñi, FM
Morero, RD
机构：
[1] Univ Nacl Tucuman, CONICET, Fac Bioquim Quim & Farm,Dept Bioquim & Nutr, Inst Quim Biol Dr B Bloj,Inst Super Invest Biol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Univ Basque Country, Dept Bioquim, CSIC, EHU,Unidad Biofis, E-48080 Bilbao, Spain
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 262卷 / 03期
关键词：
fusion; liposomes; lysozyme; glyceraldehyde-3-phosphate dehydrogenase;
D O I：
10.1006/bbrc.1999.1243
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have investigated the fusion of phospholipid vesicles induced by lysozyme and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Vesicles were composed of dimyristoylphosphatidylcholine/dioleoylphosphatidylethanolamine/cholesterol (DMPC:DOPE:Chol, 2:1:1). Small unilamellar vesicles (SUV, diameter ca. 30 nm) obtained by extensive sonication or large unilamellar vesicles (LUV, diameters ranged from 100 to 400 nm) obtained by extrusion methods were used, Fusion of LUV induced by lysozyme and GAPDH was drastically decreased when the diameter of the vesicles increased over a value of 100 nm. Lysozyme effect was stopped at the aggregation step while GAPDH effect was stopped at the fusion (lipid mixing) step. Fusion of heterogeneous vesicle populations (SUV with LW) was observed only with GAPDH and this happened only when the lipids were in the liquid-crystalline state. (C) 1999 Academic Press.
引用
收藏
页码：586 / 590
页数：5
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