High-rate biological denitrification in the cyclic rotating-bed biological reactor: Effect of COD/NO3-, nitrate concentration and salinity and the phylogenetic analysis of denitrifiers

被引：63
作者：
Jafari, Seyed Javad [1 ]
Moussavi, Gholamreza [1 ]
Yaghmaeian, Kamyar [2 ,3 ]
机构：
[1] Tarbiat Modares Univ, Dept Environm Hlth Engn, Fac Med Sci, Tehran, Iran
[2] Univ Tehran Med Sci, Sch Publ Hlth, Dept Environm Hlth Engn, Tehran, Iran
[3] Univ Tehran Med Sci, Ctr Water Qual Res, Inst Environm Res, Tehran, Iran
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 197卷
关键词：
Biological denitrification; COD/NO3-; Salinity; Cyclic reactor; Rotating bed; WASTE-WATER; MICROBIAL DIVERSITY; ELECTRON-DONOR; N2O PRODUCTION; CARBON SOURCE; PERFORMANCE; BIOFILM; REMOVAL; STRESS;
D O I：
10.1016/j.biortech.2015.08.047
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The effects of COD/NO3- ratio, nitrate concentration and salinity was tested on the performance of the CRBR in denitrification with catechol as carbon source. The maximum nitrate reduction attained at COD/NO3- ratio of 1. The CRBR operated at optimum COD/NO3- ratio could completely denitrify the nitrate at inlet concentration up to 1250 mg/L without nitrite accumulation. The maximum denitrification rate in the CRBR was 3: 56 kgNO(3)(-)/m(3) d with a nitrate reduction efficiency of 99% when the bioreactor was operated at inlet nitrate loading rate of 3: 6 kgNO(3)(-)/m(3) d. The denitrification performance of the CRBR was not affected significantly by NaCl concentrations up to 20 g/L. 16S rRNA fragment and phylogenetic analysis identified Pseudomonas resinovorans, Stenotrophomonas maltophilia and Bacillus cereus as the most abundant denitrifiers in biomass. Accordingly, the CRBR is a high-rate bioreactor and appropriate technology for treatment of nitrate-laden industrial wastewaters containing phenolic compounds and salinity. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：482 / 488
页数：7
相关论文
共 34 条
[1]   Denitrification in a packed bed biofilm reactor (BIOFOR) - Experiments with different carbon sources. [J].
Aesoy, A ;
Odegaard, H ;
Bach, K ;
Pujol, R ;
Hamon, M .
WATER RESEARCH, 1998, 32 (05) :1463-1470
[2]   Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence [J].
Anzai, Y ;
Kim, H ;
Park, JY ;
Wakabayashi, H ;
Oyaizu, H .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1563-1589
[3]  
APHA A, 2012, STANDARD METHODS EXA
[4]   Role of nutrients in the utilization of polycyclic aromatic hydrocarbons by halotolerant bacterial strain [J].
Arulazhagan, Pugazhendi ;
Vasudevan, Namsivayam .
JOURNAL OF ENVIRONMENTAL SCIENCES, 2011, 23 (02) :282-287
[5]   Effect of different salt adaptation strategies on the microbial diversity, activity, and settling of nitrifying sludge in sequencing batch reactors [J].
Bassin, Joao Paulo ;
Kleerebezem, Robbert ;
Muyzer, Gerard ;
Rosado, Alexandre Soares ;
van Loosdrecht, Mark C. M. ;
Dezotti, Marcia .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (03) :1281-1294
[6]  
Bernat K, 2003, POL J ENVIRON STUD, V12, P387
[7]   Development of cultures capable of reducing perchlorate and nitrate in high salt solutions [J].
Cang, Y ;
Roberts, DJ ;
Clifford, DA .
WATER RESEARCH, 2004, 38 (14-15) :3322-3330
[8]   Isolation and genetic identification of PAH degrading bacteria from a microbial consortium [J].
Carmen Molina, M. ;
Gonzalez, Natalia ;
Fernando Bautista, L. ;
Sanz, Raquel ;
Simarro, Raquel ;
Sanchez, Irene ;
Sanz, Jose L. .
BIODEGRADATION, 2009, 20 (06) :789-800
[9]   An efficient protocol for genomic DNA extraction fromCitrus species [J].
Yun-Jiang Cheng ;
Wen-Wu Guo ;
Hua-Lin Yi ;
Xiao-Min Pang ;
Xiuxin Deng .
Plant Molecular Biology Reporter, 2003, 21 (2) :177-178
[10]   Biodegradation and effect of formaldehyde and phenol on the denitrification process [J].
Eiroa, M ;
Vilar, A ;
Amor, L ;
Kennes, C ;
Veiga, MC .
WATER RESEARCH, 2005, 39 (2-3) :449-455
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