TNF-α MEDIATED SECRETORY COMPONENT IS POSITIVELY REGULATED BY PI3K/AKT/MTOR IN CACO-2 CELLS

被引：2
|
作者：
Liu, Dongyan [1 ,2 ]
Wang, Si
Jiang, Teng
Ding, Peng
机构：
[1] China Med Univ, Affiliated Shengjing Hosp, Res Ctr, Shenyang 110004, Liaoning Provin, Peoples R China
[2] China Med Univ, Minist Hlth, Key Lab Congenital Malformat Res, Shenyang 110004, Liaoning Provin, Peoples R China
来源：
JOURNAL OF MEDICAL BIOCHEMISTRY | 2013年 / 32卷 / 02期
基金：
中国国家自然科学基金;
关键词：
secretory component; polymeric Ig receptor; tumour necrosis factor-alpha; phosphoinositide; 3-kinase; mammalian target of rapamycin; POLYMERIC IMMUNOGLOBULIN RECEPTOR; ENTEROTOXIGENIC ESCHERICHIA-COLI; IG RECEPTOR; EXPRESSION; BINDING; PROTEIN; TRANSCYTOSIS; LACTOFERRIN; LINE;
D O I：
10.2478/jomb-2013-0008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-alpha. Methods: Caco-2 cells were cotreated with TNF-alpha plus either Wortmannin, which inhibits PI3K, or Rapamycin, which inhibits mTOR. The expression of SC and pIgR mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR. Results: Wortmannin and Rapamycin decreased the fraction of cells expressing SC, as well as the total expression of SC protein and pIgR mRNA. Conclusions: TNF-alpha regulation of SC expression is mediated through a PI3K/AKT/mTOR signaling pathway in Caco-2 cells.
引用
收藏
页码：116 / 120
页数：5
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