Epidermal growth factor receptor-coamplified and overexpressed protein (VOPP1) is a putative oncogene in gastric cancer

被引：17
作者：
Gao, Caiping [1 ,2 ]
Pang, Minghui [2 ,3 ]
Zhou, Zhou [1 ,2 ]
Long, Size [1 ,2 ]
Dong, Dandan [2 ,4 ]
Yang, Jiyun [2 ,5 ]
Cao, Mei [2 ,6 ]
Zhang, Chumin [1 ,2 ]
Han, Shengxi [1 ,2 ]
Li, Liangping [1 ,2 ]
机构：
[1] Sichuan Acad Med Sci, Dept Gastroenterol, Chengdu 610072, Sichuan Provinc, Peoples R China
[2] Sichuan Prov Peoples Hosp, Chengdu 610072, Sichuan Provinc, Peoples R China
[3] Sichuan Acad Med Sci, Dept Gastrointestinal Surg, Chengdu 610072, Sichuan, Peoples R China
[4] Sichuan Acad Med Sci, Dept Pathol, Chengdu 610072, Sichuan, Peoples R China
[5] Sichuan Acad Med Sci, Inst Lab Med, Ctr Human Mol Biol & Genet, Chengdu 610072, Sichuan, Peoples R China
[6] Sichuan Acad Med Sci, Cent Lab, Chengdu 610072, Sichuan, Peoples R China
来源：
CLINICAL AND EXPERIMENTAL MEDICINE | 2015年 / 15卷 / 04期
关键词：
ECOP; Gastric cancer; Lysosomes; Migration; Proliferation; MICRORNA DYSREGULATION; APOPTOSIS; CELLS; ECOP; MECHANISMS; EXPRESSION;
D O I：
10.1007/s10238-014-0320-7
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
MicroRNAs are found to play an important role in gastric cancer. Reduced expression of microRNA-218 (miR-218) is of key interest. The target gene of microRNA-218, epidermal growth factor receptor-coamplified and overexpressed protein (ECOP) encoded by the VOPP1 gene, has been implicated in tumorigenesis. However, few studies on expression and function of ECOP in gastric cancer have been reported. ECOP expression was determined in matched normal and gastric adenocarcinoma tissue specimens by immunohistochemistry and western blot. Subsequently, ectopic overexpression and RNAi-mediated silencing of VOPP1 was effected in the human gastric cancer cell line, AGS. Proliferation and migration of parental, VOPP1 overexpressing and VOPP1-silenced AGS cells were evaluated by cell proliferation assay and scratch wound-healing motility assay. Finally, intracellular localization of ECOP in AGS cells was assessed by green fluorescent protein tagging and fluorescent microscopy. Western blot and immunohistochemistry showed overexpression of ECOP in gastric adenocarcinoma tissues compared to matched normal tissue specimens. Ectopic overexpression and RNAi-mediated silencing of VOPP1 promoted and inhibited, respectively, cell proliferation and migration in AGS cells. Intracellular localization of ECOP in perinuclear lysosomes mimicked colocalization earlier reported for other cancerous cells. VOPP1 is overexpressed in gastric adenocarcinoma, which is involved in promoting cell proliferation and migration and thus might serve as a putative oncogene.
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收藏
页码：469 / 475
页数：7
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