EFFECT OF ANTI-TNF-α MAB ON NF-κB NUCLEAR TRANSLOCATION AND PROLIFERATION OF HUMAN HEPG2 CELLS

The activation of NF-kappa B induced by tumor necrosis factor-alpha (TNF-alpha) is closely related to hepatocarcinogenesis and drug tolerance, but the influence of inhibiting NF-kappa B Activation by TNF-alpha mAb in hepatoma cells is still poorly understood up to now. In this paper, we investigated the effect of TNF-alpha mAb on NF-kappa B activation and nuclear translocation by many methods, such as ELISA, Western Blot, qPCR, immunohistochemistry. The results indicated that the expression level of NF-kappa B mRNA was significantly inhibited. Besides, NF-kappa B activation and nuclear translocation were significantly inhibited by TNF-alpha mAb in HepG2 cells. So it can be seen that intervention with TNF-alpha mAb is expected to become a new method of HCC targeted therapy.