The Tetramer of p53 in the Absence of DNA Forms a Relaxed Quaternary State

被引：15

作者：

Nam Pham ^{[1
]}

Lucumi, Armando ^{[1
]}

Cheung, Nikki ^{[1
]}

Viadiu, Hector ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, Lab Struct Biochem, La Jolla, CA 92093 USA

来源：

BIOCHEMISTRY | 2012年 / 51卷 / 41期

基金：

美国国家卫生研究院;

关键词：

TUMOR-SUPPRESSOR; TRANSACTIVATION DOMAIN; CRYSTAL-STRUCTURE; P53-DNA COMPLEX; PROTEIN; RECOGNITION; MECHANISM; REVEALS;

D O I：

10.1021/bi301193k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

p53 is a tetrameric multidomain protein that triggers the anticancer cellular response to stress. We have calculated a three-dimensional reconstruction of full-length human p53 in the absence of DNA using single-particle electron microscopy. The reconstruction of DNA-free full-length p53 shows a square-shaped structure with four distinct domains and a hollow center. In comparison with the known compacted DNA-bound full-length p53 structures, the DNA-free p53 tetramer adopts a relaxed conformation with separated monomers and oligomerization interfaces different from those of the DNA-bound conformation.

引用

页码：8053 / 8055

页数：3

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