In Vivo Real-Time Imaging of Extracellular Vesicles in Liver Regeneration via Aggregation-Induced Emission Luminogens

被引：82
作者：
Cao, Hongmei [1 ]
Yue, Zhiwei [1 ]
Gao, Heqi [2 ]
Chen, Chao [2 ]
Cui, Kaige [1 ]
Zhang, Kaiyue [1 ]
Cheng, Yuanqiu [1 ]
Shao, Guoqiang [3 ]
Kong, Deling [2 ]
Li, Zongjin [1 ]
Ding, Dan [2 ]
Wang, Yuebing [1 ]
机构：
[1] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China
[2] Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Bioact Mat, Tianjin 300071, Peoples R China
[3] Nanjing Med Univ, Nanjing Hosp 1, Dept Nucl Med, Nanjing 210006, Jiangsu, Peoples R China
来源：
ACS NANO | 2019年 / 13卷 / 03期
基金：
中国国家自然科学基金;
关键词：
extracellular vesicles; aggregation-induced emission; human placenta-derived mesenchymal stem cells; liver regeneration; fluorescence imaging; MESENCHYMAL STEM-CELLS; REGULATORY T-CELLS; STROMAL CELLS; TRANSPLANTATION; FIBROSIS; TRACKING; INJURY;
D O I：
10.1021/acsnano.8b09776
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Extracellular vesicles (EVs) attract much attention in liver pathology because they regulate cell-cell communication and many pathophysiological events by transferring their cargos. Monitoring and understanding the in vivo fate and therapeutic capacity of these EVs is critical for the development and optimization of EV-based diagnosis and therapy. Herein, we demonstrate the use of an aggregation-induced emission luminogen, DPA-SCP, for the real-time tracking of EVs derived from human placenta-derived mesenchymal stem cells (MSCs) and their therapeutic effects in a mouse acute liver injury (ALI) model. In vitro, DPA-SCP does not alter the inherent characteristics of MSC-derived EVs and shows extremely low toxicity. Moreover, DPA-SCP exhibited superior labeling efficiency and tracking capability to the most popular commercial EV trackers, PKH26 and DiI. In vivo, DPA-SCP precisely and quantitatively tracked the behaviors of EVs for 7 days in the mouse ALI model without influencing their regenerative capacity and therapeutic efficacy. The therapeutic effects of EVs may attribute to their ability for reducing inflammatory cell infiltration, enhancing cell survival and antiapoptotic effects. In conclusion, DPA-SCP with an AIE signature serves as a favorable and safe tracker for in vivo real-time imaging of EVs in liver regeneration.
引用
收藏
页码：3522 / 3533
页数：12
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