Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters

被引：27
|
作者：
Soga, Naoki [1 ]
Watanabe, Rikiya [1 ,2 ,3 ]
Noji, Hiroyuki [1 ,3 ]
机构：
[1] Univ Tokyo, Grad Sch Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1138656, Japan
[2] Japan Sci & Technol, PRESTO, Bunkyo Ku, Tokyo 1138656, Japan
[3] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo 1020075, Japan
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
日本学术振兴会;
关键词：
ALPHA-HEMOLYSIN; ELECTROPHYSIOLOGY; RECORDINGS; CHIP;
D O I：
10.1038/srep11025
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical applications such as drug screening.
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页数：8
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