Carbon Nanocapsules as an Effective Sensing Platform for Fluorescence-Enhanced Nucleic Acid Detection

被引：4

作者：

Chang, Guohui ^{[1
]}

Hu, Jianming ^{[2
]}

Lu, Wenbo ^{[1
]}

Qin, Xiaoyun ^{[1
]}

Asiri, Abdullah M. ^{[3
,4
]}

Al-Youbi, Abdulrahman O. ^{[3
,4
]}

Sun, Xuping ^{[1
,3
,4
]}

机构：

[1] China W Normal Univ, Sch Chem & Chem Ind, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637002, Sichuan, Peoples R China

[2] Chongqing Normal Univ, Coll Phys & Elect Engn, Opt Engn Key Lab Chongqing, Chongqing 400047, Peoples R China

[3] King Abdulaziz Univ, Fac Sci, Dept Chem, Jeddah 21589, Saudi Arabia

[4] King Abdulaziz Univ, Ctr Excellence Adv Mat Res, Jeddah 21589, Saudi Arabia

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2012年 / 12卷 / 05期

关键词：

Carbon Nanocapsule; Nucleic Acid Detection; Fluorescence; DNA; PROBES; NANOPARTICLES; NANOSPHERES; NANOBELTS; POLYMERS; SOL;

D O I：

10.1166/jnn.2012.5848

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this communication, we demonstrate the proof of concept that carbon nanocapsules (CNCs) can be used as an effective fluorescent sensing platform for nucleic acid detection with selectivity down to single-base mismatch. The detection is accomplished by two steps: (1) CNC adsorbs and quenches the fluorescence of the dye-labeled single-stranded DNA (ssDNA) probe; (2) in the presence of the target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the CNC surface, leading to recovery of the dye fluorescence.

引用

页码：3775 / 3780

页数：6

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