Substitution of the precursor peptide prevents anti-prM antibody-mediated antibody-dependent enhancement of dengue virus infection

被引:6
作者
Wang, Ying [1 ,2 ]
Si, Lu-Lu [1 ]
Guo, Xiao-Lan [1 ]
Cui, Guo-hui [1 ]
Fang, Dan-Yun [1 ]
Zhou, Jun-Mei [1 ]
Yan, Hui-Jun [1 ]
Jiang, Li-Fang [1 ]
机构
[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Microbiol, Key Lab Trop Dis Control,Minist Educ China, 74 Zhongshin Rd 2, Guangzhou 510080, Guangdong, Peoples R China
[2] Weifang Med Univ, Dept Lab Med, Inst Nanomed Technol, Inst Key Lab Clin Lab Diagnost,Year Project Shand, Weifang 261053, Shandong, Peoples R China
关键词
Dengue virus; Precursor peptide; Pre-membrane; Antibody-dependent enhancement; Chimeric protein; Anti-prM antibody; JAPANESE ENCEPHALITIS; MONOCLONAL-ANTIBODIES; CONSERVED RESIDUES; HEMORRHAGIC-FEVER; IMMUNE-RESPONSE; FUSION-LOOP; PROTEIN; IDENTIFICATION; EPITOPE; GLYCOPROTEIN;
D O I
10.1016/j.virusres.2016.12.003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Antibody-dependent enhancement (ADE) is currently considered as the mechanism underlying the pathogenesis of severe dengue disease. Many studies have shown that precursor (pr) peptide-specific antibodies do not efficiently neutralize infection but potently promote ADE of dengue virus (DENV) infection. To explore the effect of pr peptide substitution on neutralization and ADE of DENV infection, the rabbit anti-prM polyclonal antibodies (pAbs) and anti-JEVpr/DENV-M pAbs were prepared, and the neutralization and ADE of these two pAbs were further compared. Here, we report that both anti-JEVpr/DENV-M and anti-prM pAbs exhibited broad cross-reactivity and only partial neutralization with four DENV serotypes and immature DENV. Rabbit anti-prM pAbs showed a significant enhancement in a broad range of serum dilutions. However, there was no statistically significant difference in the enhancing activity of rabbit anti-JEVpr/DENV-M pAbs at various levels of dilution. These results demonstrate that anti-prM antibody-mediated ADE can be prevented by JEV pr peptide replacement. The present study contribute further to research on the pathogenesis of DENV infection. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码:57 / 64
页数:8
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