Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip

被引：24

作者：

Costantini, Francesca ^{[1
]}

Tiggelaar, Roald ^{[2
]}

Sennato, Simona ^{[3
]}

Mura, Francesco ^{[4
]}

Schlautmann, Stefan ^{[2
]}

Bordi, Federico ^{[3
]}

Gardeniers, Han ^{[2
]}

Manetti, Cesare ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[2] Univ Twente, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[3] Univ Roma La Sapienza, Dipartimento Fis, CNR IPCF, I-00185 Rome, Italy

[4] Univ Roma La Sapienza, Dipartimento Sci Base Applicate All Ingn, I-00161 Rome, Italy

来源：

ANALYST | 2013年 / 138卷 / 17期

关键词：

ON-A-CHIP; HORSERADISH-PEROXIDASE; MICROFLUIDIC DEVICES; POLYMER BRUSHES; MEDICAL DIAGNOSTICS; IMMOBILIZED ENZYMES; DIABETES MANAGEMENT; MICROREACTORS; LAB; BIOSENSORS;

D O I：

10.1039/c3an00806a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 mu M. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.

引用

页码：5019 / 5024

页数：6

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