Lipopolysaccharide-induced indoleamine 2,3-dioxygenase expression in the periodontal ligament

被引:20
作者
Moon, J. S. [1 ]
Cheong, N. R. [1 ]
Yang, S. Y. [1 ]
Kim, I. S. [2 ]
Chung, H. J. [2 ]
Jeong, Y. W. [3 ]
Park, J. C. [3 ]
Kim, M. S. [1 ]
Kim, S. H. [1 ]
Ko, H. M. [3 ]
机构
[1] Chonnam Natl Univ, Med Res Ctr Biomineralizat Disorders, Dept Oral Anat,Dent Sci Res Inst, Sch Dent,Stage Brain Korea 2, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Sch Dent, Dent Sci Res Inst, Dept Periodontol, Kwangju 500757, South Korea
[3] Seonam Univ, Coll Med, Dept Microbiol, Namwon 590711, South Korea
基金
新加坡国家研究基金会;
关键词
indoleamine; 2; 3-dioxygenase; inflammation; lipopolysaccharide; periodontal ligament; TUMOR-NECROSIS-FACTOR; HUMAN GINGIVAL FIBROBLASTS; T-CELL PROLIFERATION; INTERFERON-GAMMA; PORPHYROMONAS-GINGIVALIS; GENE-EXPRESSION; DENDRITIC CELLS; IDO EXPRESSION; FACTOR-ALPHA; INHIBITION;
D O I
10.1111/jre.12063
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background and ObjectiveIndoleamine 2,3-dioxygenase (IDO) is a tryptophan-oxidizing enzyme with immune-inhibitory effects. The aim of this study was to investigate the expression of IDO by lipopolysaccharide (LPS), a component of gram-negative bacteria, in human periodontal ligament (PDL) cells. Material and MethodsHuman PDL cells and gingival fibroblasts (GFs) were prepared from explants of human PDLs and from gingival tissues of clinically healthy donors, respectively. Real-time RT-PCR, western blotting and the IDO enzyme assay were performed to determine the expression of IDO following LPS treatment of cells. LPS was injected into mice tail veins to evaluate the effects of LPS in vivo in the maxillary first molar. Immunofluorescence staining and histological analysis were followed to localize IDO in mouse PDL. ResultsThe level of expression of IDO mRNA in primary human PDL cells after LPS treatment was increased in a dose-dependent manner, reaching a peak 8h after LPS treatment. The expression and activities of IDO protein were significantly increased in comparison with those of the control. In addition, the increased production of kynurenine in culture medium was observed 72h after LPS treatment. In the immunofluorescence findings, stronger immunoreactivities were shown in PDL than in gingival tissues in the maxillae. In accordance with the immunofluorescence findings, LPS treatment induced a strong up-regulation of IDO mRNA in human PDL cells, whereas human GFs showed only a weak response to LPS. ConclusionThese results clearly show that IDO was induced by LPS in primary human PDL cells, suggesting that PDL might be involved in the regulation of oral inflammatory disease.
引用
收藏
页码:733 / 739
页数:7
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