Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA

被引：6
作者：
Petraccone, L
Erra, E
Messere, A
Montesarchio, D
Piccialli, G
De Napoli, L
Barone, G
Giancola, C
机构：
[1] Univ Naples Federico II, Dipartimento Chim, I-80126 Naples, Italy
[2] Univ Naples 2, Dipartimento Sci Ambientali, I-81100 Caserta, Italy
[3] Univ Naples Federico II, Dipartimento Chim Organ & Biochim, I-80126 Naples, Italy
[4] Dipartimento Chim Sostanze Nat, I-80131 Naples, Italy
来源：
BIOPOLYMERS | 2004年 / 73卷 / 04期
关键词：
DNA-PNA chimeras; triplex structure; circular dichroism; differential scanning calorimetry; molecular modeling;
D O I：
10.1002/bip.10599
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all-DNA complex. (C) 2004 Wiley Periodicals, Inc.
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页码：434 / 442
页数：9
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