Effect of PER1 on cell proliferation and cell migration

被引：0

作者：

Li, Shiping ^{[1
]}

Hou, Wang ^{[1
]}

Wang, Yuhui ^{[1
]}

Cheng, Shuting ^{[1
]}

Jiang, Zhou ^{[1
]}

Liu, Yanyou ^{[1
]}

Xiao, Jing ^{[1
]}

Guo, Huiling ^{[1
]}

Wang, Zhengrong ^{[1
]}

机构：

[1] Sichuan Univ, Preclin & Forens Med Sch, Key Lab Chronobiol, Minist Hlth, Chengdu 610041, Sichuan, Peoples R China

来源：

BIOLOGICAL RHYTHM RESEARCH | 2013年 / 44卷 / 02期

关键词：

circadian gene; PERIOD1; cell proliferation; MMP2; cell migration; CIRCADIAN CLOCK; MATRIX METALLOPROTEINASES; MAMMALIAN CLOCK; TUMOR-GROWTH; EXPRESSION; CANCER; GENES; PERIOD-1; BMAL1; MPER2;

D O I：

10.1080/09291016.2012.668006

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

It has been widely studied and demonstrated that circadian rhythm, as well as circadian clock genes, plays an important role in many physiological and pathological processes. Period1 (Per1) gene is a core gene in the circadian clock system that is involved in cell differentiation and apoptosis. To verify the role of PER1 on the proliferation of normal cells, we chose NIH3T3cells to have their PER1 up-regulated by transfecting a Per1 expression plasmid and determining their proliferation rates. Previous studies demonstrated that cell migration could be regulated by matrix metallopeptidase 2 (MMP2), and this MMP2 protein had a relation with the circadian system. To investigate the function of PER1 on tumor cell migration, Lewis lung carcinoma (LLC) cells were chosen to have their PER1 up-regulated, and the MMP2 level and the migration of LLC cells were tested. Our study showed that PER1 overexpression led to MMP2 down regulation in both NIH3T3 and CCL cells and inhibited the proliferation rate in NIH3T3. Migration of LLC cells could also be suppressed by the overexpression of PER1, probably by the down-regulation effect of PER1 on MMP2.

引用

页码：255 / 263

页数：9

共 29 条

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