The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of alpha-actinin

被引：66

作者：

Ohtsuka, H ^{[1
]}

Yajima, H ^{[1
]}

Maruyama, K ^{[1
]}

Kimura, S ^{[1
]}

机构：

[1] CHIBA UNIV,FAC SCI,DEPT BIOL,CHIBA 263,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 235卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.6534

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Connectin/titin, a 3000 kDa protein, links the Z line to the myosin filament in striated muscle sarcomeres. Using the yeast two-hybrid system, the present work shows that the N-terminal Z repeat 5 region (amino acids 447-472) of connectin binds to the C-terminal region (amino acids 825-897) of alpha-actinin, the main constituent of the Z line. (C) 1997 Academic Press.

引用

收藏

页码：1 / 3

页数：3

相关论文

共 13 条

[1] PRIMARY STRUCTURE OF CHICKEN SKELETAL-MUSCLE AND FIBROBLAST ALPHA-ACTININS DEDUCED FROM CDNA SEQUENCES [J].

ARIMURA, C ;

SUZUKI, T ;

YANAGISAWA, M ;

IMAMURA, M ;

HAMADA, Y ;

MASAKI, T .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (03) :649-655

[2] ASSOCIATION OF INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) WITH ACTIN-CONTAINING CYTOSKELETON AND ALPHA-ACTININ [J].

CARPEN, O ;

PALLAI, P ;

STAUNTON, DE ;

SPRINGER, TA .

JOURNAL OF CELL BIOLOGY, 1992, 118 (05) :1223-1234

[3] ALPHA-ACTININ A NEW STRUCTURAL PROTEIN FROM STRIATED MUSCLE .I. PREPARATION AND ACTION ON ACTOMYOSIN-ATP INTERACTION [J].

EBASHI, S ;

EBASHI, F .

JOURNAL OF BIOCHEMISTRY, 1965, 58 (01) :7-&

[4]

Gautel M, 1996, J CELL SCI, V109, P2747

[5] TITINS - GIANT PROTEINS IN CHARGE OF MUSCLE ULTRASTRUCTURE AND ELASTICITY [J].

LABEIT, S ;

KOLMERER, B .

SCIENCE, 1995, 270 (5234) :293-296

[6] CONNECTIN, AN ELASTIC PROTEIN OF STRIATED-MUSCLE [J].

MARUYAMA, K .

BIOPHYSICAL CHEMISTRY, 1994, 50 (1-2) :73-85

[7]

MARUYAMA K, 1965, J BIOCH, V58, P20

[8]

MARUYAMA K, 1997, IN PRESS FASEB J

[9] LOCALIZATION OF 6S COMPONENT OF ALPHA-ACTININ AT Z-BAND [J].

MASAKI, T ;

ENDO, M ;

EBASHI, S .

JOURNAL OF BIOCHEMISTRY, 1967, 62 (05) :630-&

[10] Binding of the N-terminal 63 kDa portion of connectin/titin to alpha-actinin as revealed by the yeast two-hybrid system [J].

Ohtsuka, H ;

Yajima, H ;

Maruyama, K ;

Kimura, S .

FEBS LETTERS, 1997, 401 (01) :65-67