Function of Cytochrome P450 Enzymes RosC and RosD in the Biosynthesis of Rosamicin Macrolide Antibiotic Produced by Micromonospora rosaria

被引：17

作者：

Iizaka, Yohei ^{[1
]}

Higashi, Noriko ^{[1
]}

Ishida, Masanari ^{[1
]}

Oiwa, Reina ^{[1
]}

Ichikawa, Yumi ^{[1
]}

Takeda, Moeka ^{[1
]}

Anzai, Yojiro ^{[1
]}

Kato, Fumio ^{[1
]}

机构：

[1] Toho Univ, Fac Pharmaceut Sci, Funabashi, Chiba 274, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2013年 / 57卷 / 03期

关键词：

ESCHERICHIA-COLI; STREPTOMYCES; GENE; TYLOSIN; PATHWAY; CLONING;

D O I：

10.1128/AAC.02092-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cytochrome P450 enzyme-encoding genes rosC and rosD were cloned from the rosamicin biosynthetic gene cluster of Micromonospora rosaria IFO13697. The functions of RosC and RosD were demonstrated by gene disruption and complementation with M. rosaria and bioconversion of rosamicin biosynthetic intermediates with Escherichia coli expressing RosC and RosD. It is proposed that M. rosaria IFO13697 has two pathway branches that lead from the first desosaminyl rosamicin intermediate, 20-deoxo-20-dihydro-12,13-deepoxyrosamicin, to rosamicin.

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页码：1529 / 1531

页数：3

相关论文

共 16 条

[1] Hydroxylation of testosterone by bacterial cytochromes P450 using the Escherichia coli expression system [J].

Agematu, H ;

Matsumoto, N ;

Fujii, Y ;

Kabumoto, H ;

Doi, S ;

Machida, K ;

Ishikawa, J ;

Arisawa, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2006, 70 (01) :307-311

[2] Isolation and characterization of 23-O-mycinosyl-20-dihydro-rosamicin: a new rosamicin analogue derived from engineered Micromonospora rosaria [J].

Anzai, Yojiro ;

Sakai, Ayami ;

Li, Wei ;

Iizaka, Yohei ;

Koike, Kazuo ;

Kinoshita, Kenji ;

Kato, Fumio .

JOURNAL OF ANTIBIOTICS, 2010, 63 (06) :325-328

[3] Production of rosamicin derivatives in Micromonospora rosaria by introduction of d-mycinose biosynthetic gene with I¦C31-derived integration vector pSET152 [J].

Anzai, Yojiro ;

Iizaka, Yohei ;

Li, Wei ;

Idemoto, Naoki ;

Tsukada, Shu-ichi ;

Koike, Kazuo ;

Kinoshita, Kenji ;

Kato, Fumio .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2009, 36 (08) :1013-1021

[4] BIOSYNTHESIS OF THE MACROLIDE ANTIBIOTIC TYLOSIN - A PREFERRED PATHWAY FROM TYLACTONE TO TYLOSIN [J].

BALTZ, RH ;

SENO, ET ;

STONESIFER, J ;

WILD, GM .

JOURNAL OF ANTIBIOTICS, 1983, 36 (02) :131-141

[5] PLASMID CLONING VECTORS FOR THE CONJUGAL TRANSFER OF DNA FROM ESCHERICHIA-COLI TO STREPTOMYCES SPP [J].

BIERMAN, M ;

LOGAN, R ;

OBRIEN, K ;

SENO, ET ;

RAO, RN ;

SCHONER, BE .

GENE, 1992, 116 (01) :43-49

[6] Cloning and characterization of genes encoded in dTDP-D-mycaminose biosynthetic pathway from a midecamycin-producing strain, Streptomyces mycarofaciens [J].

Cong, Lina ;

Piepersberg, Wolfgang .

ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2007, 39 (03) :187-193

[7]

FARNET CM, 2003, Patent No. 03010193

[8] PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin [J].

Gust, B ;

Challis, GL ;

Fowler, K ;

Kieser, T ;

Chater, KF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :1541-1546

[9] Organization of the biosynthetic gene cluster for the macrolide antibiotic spiramycin in Streptomyces ambofaciens [J].

Karray, Fatma ;

Darbon, Emmanuelle ;

Oestreicher, Nathahe ;

Dominguez, Helene ;

Tuphile, Karine ;

Gagnat, Josette ;

Blondelet-Rouault, Marie-Helene ;

Pernodet, Claude Gerbaud Jean-Luc .

MICROBIOLOGY-SGM, 2007, 153 :4111-4122

[10] MACROLIDE ANTIBIOTICS M-4365 PRODUCED BY MICROMONOSPORA .2. CHEMICAL STRUCTURES [J].

KINUMAKI, A ;

HARADA, K ;

SUZUKI, T ;

SUZUKI, M ;

OKUDA, T .

JOURNAL OF ANTIBIOTICS, 1977, 30 (06) :450-454